Szenberg A
Adv Exp Med Biol. 1979;114:157-64. doi: 10.1007/978-1-4615-9101-6_23.
Using fowl anti mouse (Fab)2 antibody, a protein has been collected from culture fluid and cell lysates of thymoma cells and stimulated thymocytes. This protein did show an immunoglobulin arc on immunoelectrophoresis against abovementioned antibody and against rabbit anti mouse Ig serum. No precipitation lines have been produced by mono-specific anti-mouse gamma, mu and alpha sera. On reduced PAGE heavy and light chains were present. Mobility of the heavy chain was between mu and gamma chains and its apparent molecular weight was calculated at appr. 65,000 daltons. The light chain mobility corresponded to mouse serum kappa chains.
使用鸡抗小鼠(Fab)2抗体,从胸腺瘤细胞和经刺激的胸腺细胞的培养液及细胞裂解物中收集到一种蛋白质。该蛋白质在针对上述抗体以及兔抗小鼠Ig血清的免疫电泳中确实显示出一条免疫球蛋白弧。单特异性抗小鼠γ、μ和α血清未产生沉淀线。在还原聚丙烯酰胺凝胶电泳上存在重链和轻链。重链的迁移率介于μ链和γ链之间,其表观分子量经计算约为65,000道尔顿。轻链迁移率与小鼠血清κ链相对应。