Yarmush M L, Gates F T, Dreher K L, Kindt T J
J Immunol. 1981 Jun;126(6):2240-4.
Serologic and primary structural analyses of Ig chains secreted by several rabbit-mouse hybridomas have shown that these hybrid cells produce heavy (H) or light (L) chains identical to those isolated from rabbit sera. Two of the cell lines (7D2, 7D6) secreted rabbit H chains with a m.w. of 55,000 each of which expressed a full complement of variable and constant region allotypes (a3, d11, e15). These apparently normal rabbit H chains were secreted in a complex with a m.w. about 130,000, and serologic studies indicated that this complex contained a covalently linked mouse kappa L chain. Two other cell lines (4C1, 12F2) produced allotype b4 L chains with m.w. of 23,000 and 25,000, and a third (1D4P5) produced an allotype b5 L chain with a m.w. of 23,000. Serologic analyses indicated that the allotypes on these chains are equivalent to those expressed by normal rabbit Ig molecules. Partial amino acid sequence data obtained for the L chain products showed them to be typical of rabbit L chains, and to be significantly different from mouse L chains.
对几种兔 - 鼠杂交瘤分泌的免疫球蛋白（Ig）链进行的血清学和一级结构分析表明，这些杂交细胞产生的重链（H）或轻链（L）与从兔血清中分离出的链相同。其中两个细胞系（7D2、7D6）分泌分子量为55,000的兔重链，每条重链都表达了全套可变区和恒定区同种异型（a3、d11、e15）。这些明显正常的兔重链以分子量约为130,000的复合物形式分泌，血清学研究表明该复合物包含一条共价连接的小鼠κ轻链。另外两个细胞系（4C1、12F2）产生分子量分别为23,000和25,000的同种异型b4轻链，第三个细胞系（1D4P5）产生分子量为23,000的同种异型b5轻链。血清学分析表明，这些链上的同种异型与正常兔Ig分子表达的同种异型相同。对轻链产物获得的部分氨基酸序列数据显示，它们是典型的兔轻链，与小鼠轻链有显著差异。
