Shinoda K, Ishida S, Kawashima S, Wakabayashi T, Uchita M, Matsuzaki T, Takayama M, Shinmura K, Yamada M
Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
Curr Eye Res. 2000 Aug;21(2):655-61.
To investigate clinical factors related to the aqueous humor levels of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) in patients with proliferative diabetic retinopathy (PDR).
Undiluted aqueous humor was obtained during ocular surgery from 46 eyes of 46 patients and the levels of growth factors were measured using enzyme-linked immunosorbent assays. VEGF and HGF levels were compared with the number of photocoagulation. VEGF and HGF levels in patient groups stratified according to the existence of vitreous hemorrhage, retinal detachment, and fibrovascular membrane were analyzed. And clinical parameters associated with the growth factors were determined by multiple regression analysis.
The levels of VEGF decreased significantly as the extent of photocoagulation increased and showed significant positive correlation with the existence of vitreous hemorrhage. There was no significant correlation between VEGF levels and the existence of fibrovascular membrane and traction retinal detachment. The levels of HGF also decreased significantly as the extent of photocoagulation increased, but increased significantly when fibrovascular membrane existed. There were no significant correlations between HGF levels and the existence of vitreous hemorrhage and traction retinal detachment. Concerning systemic conditions, each growth factor has no significant correlation with duration and type of diabetes mellitus, the treatment regimen, the control of hemoglobin A1C, and the existence of hypertension or renal dysfunction.
The therapeutic effect of panretinal photocoagulation on PDR might be partly exerted by reduction of the levels of VEGF and HGF in ocular fluid. Since the clinical parameters associated with VEGF were different from those associated with HGF, these growth factors might influence the progression of retinopathy in different ways.
研究增殖性糖尿病视网膜病变（PDR）患者房水中血管内皮生长因子（VEGF）和肝细胞生长因子（HGF）水平相关的临床因素。
在眼科手术中从46例患者的46只眼中获取未稀释的房水，采用酶联免疫吸附测定法测量生长因子水平。将VEGF和HGF水平与光凝次数进行比较。分析根据玻璃体出血、视网膜脱离和纤维血管膜的存在进行分层的患者组中的VEGF和HGF水平。通过多元回归分析确定与生长因子相关的临床参数。
随着光凝范围的增加，VEGF水平显著降低，并且与玻璃体出血的存在呈显著正相关。VEGF水平与纤维血管膜和牵拉性视网膜脱离的存在之间无显著相关性。随着光凝范围的增加，HGF水平也显著降低，但在存在纤维血管膜时显著升高。HGF水平与玻璃体出血和牵拉性视网膜脱离的存在之间无显著相关性。关于全身状况，每种生长因子与糖尿病的病程和类型、治疗方案、糖化血红蛋白A1C的控制以及高血压或肾功能不全的存在均无显著相关性。
全视网膜光凝对PDR的治疗作用可能部分是通过降低眼液中VEGF和HGF的水平来实现的。由于与VEGF相关的临床参数不同于与HGF相关的临床参数，这些生长因子可能以不同方式影响视网膜病变的进展。
