Togneri A M, Cimalando L
Laboratorio de Bacteriología, Hospital Interzonal General de Agudos Evita, Río de Janeiro 1910, 1824 Lanús, Pcia. de Buenos Aires, Argentina.
Rev Argent Microbiol. 2000 Oct-Dec;32(4):173-8.
The advantages of Mycobacteria Growth Indicator Tube (MGIT) system were analyzed and compared to Löwenstein-Jensen (LJ) and Stonebrink (S) solid media when searching for a fast method to diagnose tuberculosis and mycobacterioses, which should be easy to perform in laboratories and of non-invasive reading. All nonsterile specimens were pretreated with Petroff method. A total of 191 specimens were processed (among which 152 were pulmonary and 39 extrapulmonary). Twenty-nine tested positive by one of the methods employed. The rate of recovery of smear positive specimens (ED+) was of 92% with LJ/S, and 85% with MGIT. The mean time to detect a positive result was 18.7 days with MGIT, and 20.6 days with LJ/S. The rate of recovery of smear negative specimens (ED-) was 88.2% with LJ, 70.6% with S, and 23.5% with MGIT. The mean time to detect a positive result were 38.5; 26.5 and 29 days, respectively. During this experiment, MGIT did not seem to have any advantage over the traditional methods, particularly when working with samples containing a low number of bacilli (p < 0.05). It is obviously necessary to make a comparative study with a large amount of cases which might support this observation and to analyze the influence of the reagents employed in the pre-treatment of the specimens on the MGIT system's efficacy to detect mycobacteria.
在寻找一种快速诊断结核病和分枝杆菌病的方法时，分析并比较了分枝杆菌生长指示管（MGIT）系统与罗-琴（LJ）和斯通布林克（S）固体培养基的优势，该方法应易于在实验室操作且读数无需侵入性操作。所有非无菌标本均采用彼得罗夫方法进行预处理。共处理了191份标本（其中152份为肺部标本，39份为肺外标本）。通过所采用的方法之一检测出29份呈阳性。涂片阳性标本（ED+）的回收率在LJ/S培养基上为92%，在MGIT系统上为85%。MGIT检测出阳性结果的平均时间为18.7天，LJ/S培养基为20.6天。涂片阴性标本（ED-）的回收率在LJ培养基上为88.2%，在S培养基上为70.6%，在MGIT系统上为23.5%。检测出阳性结果的平均时间分别为38.5天、26.5天和29天。在该实验中，MGIT似乎没有比传统方法具有任何优势，特别是在处理含菌量低的样本时（p<0.05）。显然有必要进行大量病例的对比研究以支持这一观察结果，并分析标本预处理中所用试剂对MGIT系统检测分枝杆菌效力的影响。
