半子囊菌酵母的基因组探索：5. 葡萄汁酵母变种。

Genomic exploration of the hemiascomycetous yeasts: 5. Saccharomyces bayanus var. uvarum.

作者信息

Bon E, Neuvéglise C, Casaregola S, Artiguenave F, Wincker P, Aigle M, Durrens P

机构信息

Laboratoire de Biologie Cellulaire de la Levure, UMR 5095, Bordeaux, France.

出版信息

FEBS Lett. 2000 Dec 22;487(1):37-41. doi: 10.1016/s0014-5793(00)02276-6.

DOI:10.1016/s0014-5793(00)02276-6

PMID:11152880

Abstract

Saccharomyces bayanus var. uvarum investigated here is the species closest to Saccharomyces cerevisiae. Random sequence tags (RSTs) allowed us to identify homologues to 2789 open reading frames (ORFs) in S. cerevisiae, ORFs duplicated in S. uvarum but not in S. cerevisiae, centromeres, tRNAs, homologues of Ty1/2 and Ty4 retrotransposons, and a complete rDNA repeat. Only 13 RSTs seem to be homologous to sequences in other organisms but not in S. cerevisiae. As the synteny between the two species is very high, cases in which synteny is lost suggest special mechanisms of genome evolution. The corresponding RSTs revealed that S. uvarum can exist without any S. cerevisiae DNA introgression. Accession numbers are from AL397139 to AL402278 in the EMBL databank.

摘要

这里所研究的巴氏酵母变种葡萄汁酵母是最接近酿酒酵母的物种。随机序列标签（RST）使我们能够鉴定出酿酒酵母中2789个开放阅读框（ORF）的同源物、在葡萄汁酵母中重复但在酿酒酵母中未重复的ORF、着丝粒、tRNA、Ty1/2和Ty4反转录转座子的同源物以及完整的核糖体DNA重复序列。只有13个RST似乎与酿酒酵母以外的其他生物中的序列同源。由于这两个物种之间的共线性非常高，共线性丧失的情况表明了基因组进化的特殊机制。相应的RST表明，葡萄汁酵母可以在没有任何酿酒酵母DNA渗入的情况下存在。登录号来自EMBL数据库中的AL397139至AL402278。