Bryantseva I A, Gorlenko V M, Kompantseva E I, Imhoff J F
Int J Syst Evol Microbiol. 2000 Nov;50 Pt 6:2157-2163. doi: 10.1099/00207713-50-6-2157.
Four strains of purple sulfur bacteria containing bacteriochlorophyll b were isolated from cyanobacterial mats of soda lakes in the steppe of south-east Siberia, Russia. Cells of all strains were cocci without gas vesicles. Eventually, cells with flagella were seen in the electron microscope, but motile cells were observed very rarely in cultures. Internal photosynthetic membranes were of the tubular type. Photosynthetic pigments were bacteriochlorophyll b and carotenoids with spectral characteristics similar to 3,4,3',4'-tetrahydrospirilloxanthin. The bacteria were obligately phototrophic and strictly anaerobic. Hydrogen sulfide and elemental sulfur were used as photosynthetic electron donors. Thiosulfate was not used. During growth on sulfide, sulfur globules were formed as intermediate oxidation products, deposited inside the cells and centrally located. In the presence of sulfide and sodium bicarbonate, acetate, malate, propionate, pyruvate, succinate, fumarate and yeast extract were photoassimilated. Growth factors were not required. The new bacterium is an obligate alkaliphile growing at pH 8-10 with an optimum at pH 9. It showed good growth up to 6.0% sodium chloride and up to 8.5% sodium carbonates. Phenotypically, it is similar to Thiococcus pfennigii, but different by virtue of its alkaliphily and salt tolerance. The DNA G+C content was 63.6-64.8 mol %, compared to 69.4-69.9 mol % for Thiococcus pfennigii. The 16S rDNA sequence of strain A26T was approximately 92% similar to that of Thiococcus pfennigii DSM 226 and therefore a new genus and species name, Thioalkalicoccus limnaeus gen. nov. and sp. nov., are proposed for the new bacterium.
从俄罗斯西伯利亚东南部草原苏打湖的蓝藻席中分离出了四株含细菌叶绿素b的紫色硫细菌。所有菌株的细胞均为球菌，无气泡。最终，在电子显微镜下观察到了有鞭毛的细胞，但在培养物中很少观察到运动细胞。内部光合膜为管状。光合色素为细菌叶绿素b和类胡萝卜素，其光谱特征与3,4,3',4'-四氢螺旋藻黄素相似。这些细菌为专性光养菌且严格厌氧。硫化氢和元素硫用作光合电子供体，不利用硫代硫酸盐。在硫化物上生长期间，硫球作为中间氧化产物形成，沉积在细胞内并位于中央。在有硫化物和碳酸氢钠存在的情况下，乙酸盐、苹果酸盐、丙酸盐、丙酮酸盐、琥珀酸盐、富马酸盐和酵母提取物可被光同化。不需要生长因子。这种新细菌是一种专性嗜碱菌，在pH 8-10生长，最适pH为9。它在高达6.0%的氯化钠和高达8.5%的碳酸钠中生长良好。从表型上看，它与芬尼硫球菌相似，但因其嗜碱性和耐盐性而有所不同。DNA的G+C含量为63.6-64.8 mol%，而芬尼硫球菌为69.4-69.9 mol%。菌株A26T的16S rDNA序列与芬尼硫球菌DSM 226的序列相似度约为92%，因此为这种新细菌提出了一个新的属名和种名，即湖生硫碱球菌属新属及新种。
