Caumette Pierre, Guyoneaud Remy, Imhoff Johannes F, Süling Jörg, Gorlenko Vladimir
Laboratoire d'Ecologie Moléculaire, EA 3525, IBEAS, Université de Pau et des Pays de l'Adour, BP1155, F-64013 Pau Cedex, France.
Institut für Meereskunde an der Universität Kiel, Abteilung Marine Mikrobiologie, Düsternbrooker Weg 20, D-24105 Kiel, Germany.
Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1031-1036. doi: 10.1099/ijs.0.02964-0.
Four marine, phototrophic, purple sulfur bacteria (strains 5811T, 5812, BM-3 and BS-1) were isolated in pure culture from different brackish to marine sediments in the Mediterranean Sea, the White Sea and the Black Sea. Single cells of these strains were coccus-shaped, non-motile and did not contain gas vesicles. The colour of cell suspensions that were grown in the light was purple-red. Bacteriochlorophyll a and carotenoids of the okenone series were present as photosynthetic pigments. Photosynthetic membrane systems were of the vesicular type. Hydrogen sulfide, thiosulfate, elemental sulfur and molecular hydrogen were used as electron donors during photolithotrophic growth under anoxic conditions; carbon dioxide was utilized as the carbon source. During growth on sulfide, elemental sulfur globules were stored inside the cells. In the presence of hydrogen sulfide, several organic substances could be photoassimilated. Comparative 16S rDNA sequence analysis revealed an affiliation of these four strains to the genus Thiocapsa. Both phylogenetic analysis and the results of DNA-DNA hybridization studies revealed that these strains formed a separate cluster within the genus Thiocapsa. Thus, according to phenotypic characteristics and mainly the carotenoid composition, 16S rDNA sequence analysis and DNA-DNA hybridization data, it is proposed that these strains should be classified as a novel species, Thiocapsa marina sp. nov., with strain 5811T (=DSM 5653T=ATCC 43172T) as the type strain.
从地中海、白海和黑海不同的咸淡水至海洋沉积物中，通过纯培养分离出了四株海洋光合紫色硫细菌（菌株5811T、5812、BM - 3和BS - 1）。这些菌株的单细胞呈球菌状，无运动性，且不含有气体泡囊。在光照下培养的细胞悬液颜色为紫红色。光合色素包括细菌叶绿素a和奥克酮系列类胡萝卜素。光合膜系统为泡囊型。在缺氧条件下进行光无机营养生长时，硫化氢、硫代硫酸盐、元素硫和分子氢用作电子供体；二氧化碳用作碳源。在以硫化物生长期间，细胞内会储存元素硫球。在有硫化氢存在的情况下，几种有机物质可被光同化。比较16S rDNA序列分析表明这四株菌株属于硫帽菌属。系统发育分析和DNA - DNA杂交研究结果均表明，这些菌株在硫帽菌属内形成了一个单独的聚类。因此，根据表型特征，主要是类胡萝卜素组成、16S rDNA序列分析和DNA - DNA杂交数据，建议将这些菌株分类为一个新物种，即海洋硫帽菌（Thiocapsa marina sp. nov.），以菌株5811T（=DSM 5653T =ATCC 43172T）作为模式菌株。
