起始密码子影响带有或不带有5'非翻译前导序列的cI mRNA在大肠杆菌中的核糖体结合及翻译效率。
The initiation codon affects ribosome binding and translational efficiency in Escherichia coli of cI mRNA with or without the 5' untranslated leader.
作者信息
O'Donnell S M, Janssen G R
机构信息
Department of Microbiology, Miami University, Oxford, Ohio 45056, USA.
出版信息
J Bacteriol. 2001 Feb;183(4):1277-83. doi: 10.1128/JB.183.4.1277-1283.2001.
Abstract
Translational efficiency of an AUG, CUG, GUG, or UUG initiation codon was measured for the naturally leaderless cI mRNA from bacteriophage lambda. In a cI-lacZ translational fusion, only AUG supported a high level of expression; GUG supported a low level of expression, while UUG and CUG expression was barely above background levels. Addition of an untranslated lac leader and Shine-Dalgarno sequence to cI increased expression but still showed a dependence on an AUG for maximum expression. cI-lacZ mRNA with an AUG initiation codon showed a greater in vitro ribosome binding strength and a higher level of full-length in vivo mRNA, suggesting that the initiation codon is an important determinant of ribosome binding strength and translational efficiency for mRNA with or without the 5' untranslated leader.
摘要
针对来自噬菌体λ的天然无前导序列的cI mRNA，测定了AUG、CUG、GUG或UUG起始密码子的翻译效率。在cI-lacZ翻译融合体中，只有AUG支持高水平表达；GUG支持低水平表达，而UUG和CUG的表达仅略高于背景水平。向cI添加未翻译的lac前导序列和Shine-Dalgarno序列可增加表达，但最大表达仍依赖于AUG。具有AUG起始密码子的cI-lacZ mRNA在体外显示出更强的核糖体结合强度以及在体内具有更高水平的全长mRNA，这表明起始密码子是决定有无5'非翻译前导序列的mRNA的核糖体结合强度和翻译效率的重要因素。
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