起始密码子与无 5' 端引导序列的 mRNA 之间的接近程度是影响大肠杆菌核糖体结合和表达的一个强烈的积极决定因素。
Proximity of the start codon to a leaderless mRNA's 5' terminus is a strong positive determinant of ribosome binding and expression in Escherichia coli.
机构信息
Department of Microbiology, Miami University, Oxford, Ohio 45056, USA.
出版信息
J Bacteriol. 2010 Dec;192(24):6482-5. doi: 10.1128/JB.00756-10. Epub 2010 Oct 22.
Abstract
An AUG start codon is an important determinant of ribosome binding and expression of leaderless mRNAs in Escherichia coli. Using reporter constructs encoding mRNAs where the AUG start codon is preceded by untranslated leaders of various length and sequence, we find that close proximity of the start codon to the 5' terminus and the leader sequence are strong determinants of both ribosome binding and expression.
摘要
AUG 起始密码子是大肠杆菌中核糖体结合和无引导 mRNA 表达的重要决定因素。使用报告基因构建体，我们发现，在编码 mRNA 的 AUG 起始密码子之前，具有不同长度和序列的非翻译先导序列，该起始密码子与 5'末端和先导序列的紧密接近是核糖体结合和表达的重要决定因素。
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2
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3
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