GUG和AUG起始密码子对大肠杆菌中lacZ表达的影响。

Effects of GUG and AUG initiation codons on the expression of lacZ in Escherichia coli.

作者信息

Looman A C, van Knippenberg P H

出版信息

FEBS Lett. 1986 Mar 3;197(1-2):315-20. doi: 10.1016/0014-5793(86)80349-0.

PMID:2419166

Abstract

We have replaced the ribosomal binding site (RBS) of the lacZ gene of E. coli by those of the maturation (A) gene of phage MS2 and that of the tufA gene. Both RBSs contain a GUG initiation codon. The expression with the tufA RBS is at least 25-fold higher than with the phage RBS. Changing the GUG into AUG results in a 3-fold increase in expression in both cases. In general, higher expression is accompanied by an increase of lac-specific mRNA. It is argued that this is a consequence of the more efficient translation of the mRNA.

摘要

我们已将大肠杆菌lacZ基因的核糖体结合位点（RBS）替换为噬菌体MS2成熟（A）基因和tufA基因的核糖体结合位点。两个RBS均包含GUG起始密码子。tufA RBS的表达比噬菌体RBS的表达至少高25倍。在两种情况下，将GUG变为AUG都会使表达增加3倍。一般来说，较高的表达伴随着lac特异性mRNA的增加。有人认为这是mRNA翻译效率更高的结果。