在血清群O20的基因多样化产肠毒素大肠杆菌分离株中鉴定出携带一种新粘附素的菌株簇。
Identification of a cluster of strains bearing a new adhesin among genetically diverse enterotoxigenic Escherichia coli isolates of serogroup O20.
作者信息
Pichel M, Binsztein N, Gutkind G, Viboud G
机构信息
Departamento de Bacteriología, Instituto Nacional de Enfermedades Infecciosas-ANLIS Carlos G. Malbrán, Buenos Aires, Argentina.
出版信息
J Clin Microbiol. 2001 Feb;39(2):782-6. doi: 10.1128/JCM.39.2.782-786.2001.
Abstract
About one-third of the enterotoxigenic Escherichia coli isolates lack any of the known colonization factors. Among this group, those of serogroup O20 are the most frequently isolated in Argentina. By combining analysis of adhesion to Caco-2 cells, random amplified polymorphic DNA, and pulsed-field gel electrophoresis techniques, we were able to identify three sets of closely related strains with different binding properties. Further analysis of the most prevalent group revealed that all these isolates expressed the recently described adhesin CS22.
摘要
约三分之一的产肠毒素大肠杆菌分离株缺乏任何已知的定植因子。在这一组中，O20血清群的分离株在阿根廷最常被分离到。通过结合对Caco-2细胞的黏附分析、随机扩增多态性DNA分析和脉冲场凝胶电泳技术，我们能够鉴定出三组具有不同结合特性的密切相关菌株。对最普遍的一组菌株的进一步分析表明，所有这些分离株都表达了最近描述的黏附素CS22。
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