Nogueira C, Figueiredo C, Carneiro F, Gomes A T, Barreira R, Figueira P, Salgado C, Belo L, Peixoto A, Bravo J C, Bravo L E, Realpe J L, Plaisier A P, Quint W G, Ruiz B, Correa P, van Doorn L J
Institute of Molecular Pathology and Immunology and the Faculty of Medicine, University of Porto, Porto, Portugal.
Am J Pathol. 2001 Feb;158(2):647-54. doi: 10.1016/s0002-9440(10)64006-0.
The outcome of Helicobacter pylori infection has been associated with specific virulence-associated bacterial genotypes. The present study aimed to investigate the gastric histopathology in Portuguese and Colombian patients infected with H. pylori and to assess its relationship with bacterial virulence-associated vacA, cagA, and iceA genotypes. A total of 370 patients from Portugal (n = 192) and Colombia (n = 178) were studied. Corpus and antrum biopsy specimens were collected from each individual. Histopathological features were recorded and graded according to the updated Sydney system. H. pylori vacA, cagA, and iceA genes were directly genotyped in the gastric biopsy specimens by polymerase chain reaction and reverse hybridization. Despite the significant differences between the Portuguese and Colombian patient groups, highly similar results were observed with respect to the relation between H. pylori genotypes and histopathology. H. pylori vacA s1, vacA m1, cagA+ genotypes were significantly associated with a higher H. pylori density, higher degrees of lymphocytic and neutrophilic infiltrates, atrophy, the type of intestinal metaplasia, and presence of epithelial damage. The iceA1 genotype was only associated with epithelial damage in Portuguese patients. These findings show that distinct H. pylori genotypes are strongly associated with histopathological findings in the stomach, confirming their relevance for the development of H. pylori-associated gastric pathology.
幽门螺杆菌感染的结果与特定的毒力相关细菌基因型有关。本研究旨在调查葡萄牙和哥伦比亚幽门螺杆菌感染患者的胃组织病理学情况，并评估其与细菌毒力相关的vacA、cagA和iceA基因型之间的关系。共对来自葡萄牙的192名患者和来自哥伦比亚的178名患者进行了研究。从每个个体采集胃体和胃窦活检标本。根据更新后的悉尼系统记录并分级组织病理学特征。通过聚合酶链反应和反向杂交对胃活检标本中的幽门螺杆菌vacA、cagA和iceA基因进行直接基因分型。尽管葡萄牙和哥伦比亚患者组之间存在显著差异，但在幽门螺杆菌基因型与组织病理学之间的关系方面观察到了高度相似的结果。幽门螺杆菌vacA s1、vacA m1、cagA+基因型与较高的幽门螺杆菌密度、较高程度的淋巴细胞和中性粒细胞浸润、萎缩、肠化生类型以及上皮损伤的存在显著相关。iceA1基因型仅与葡萄牙患者的上皮损伤有关。这些发现表明，不同的幽门螺杆菌基因型与胃组织病理学结果密切相关，证实了它们在幽门螺杆菌相关胃部病变发展中的相关性。
