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细胞内颗粒的动力辅助运输模型。

Models of motor-assisted transport of intracellular particles.

作者信息

Smith D A, Simmons R M

机构信息

The Randall Centre for Molecular Mechanisms of Cell Function, King's College London, Guy's Campus, London SE1 1UL, United Kingdom.

出版信息

Biophys J. 2001 Jan;80(1):45-68. doi: 10.1016/S0006-3495(01)75994-2.

Abstract

One-dimensional models are presented for the macroscopic intracellular transport of vesicles and organelles by molecular motors on a network of aligned intracellular filaments. A motor-coated vesicle or organelle is described as a diffusing particle binding intermittently to filaments, when it is transported at the motor velocity. Two models are treated in detail: 1) a unidirectional model, where only one kind of motor is operative and all filaments have the same polarity; and 2) a bidirectional model, in which filaments of both polarities exist (for example, a randomly polarized actin network for myosin motors) and/or particles have plus-end and minus-end motors operating on unipolar filaments (kinesin and dynein on microtubules). The unidirectional model provides net particle transport in the absence of a concentration gradient. A symmetric bidirectional model, with equal mixtures of filament polarities or plus-end and minus-end motors of the same characteristics, provides rapid transport down a concentration gradient and enhanced dispersion of particles from a point source by motor-assisted diffusion. Both models are studied in detail as a function of the diffusion constant and motor velocity of bound particles, and their rates of binding to and detachment from filaments. These models can form the basis of more realistic models for particle transport in axons, melanophores, and the dendritic arms of melanocytes, in which networks of actin filaments and microtubules coexist and motors for both types of filament are implicated.

摘要

本文提出了一维模型,用于描述分子马达在排列好的细胞内细丝网络上对囊泡和细胞器进行宏观细胞内运输的过程。当一个由马达蛋白包裹的囊泡或细胞器以马达速度运输时,它被描述为一个在细丝上间歇性结合的扩散粒子。本文详细讨论了两种模型:1)单向模型,其中只有一种马达蛋白起作用,且所有细丝具有相同的极性;2)双向模型,其中存在两种极性的细丝(例如,用于肌球蛋白马达的随机极化肌动蛋白网络)和/或粒子在单极细丝上同时具有向正极和向负极运动的马达蛋白(微管上的驱动蛋白和动力蛋白)。单向模型在没有浓度梯度的情况下提供净粒子运输。对称双向模型具有相同数量的两种极性细丝或相同特性的向正极和向负极马达蛋白,该模型可实现沿浓度梯度的快速运输,并通过马达辅助扩散增强粒子从点源的扩散。本文详细研究了这两种模型,分析了其作为结合粒子的扩散常数、马达速度以及它们与细丝结合和解离速率的函数关系。这些模型可以作为轴突、黑素细胞和黑素细胞树突臂中粒子运输更现实模型的基础,在这些结构中,肌动蛋白细丝和微管网络共存,且两种类型细丝的马达蛋白都有涉及。

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