Shungskaia V E, Riabov S I, Enenko S O, Pylaev A S
Tsitologiia. 1979 Nov;21(11):1326-30.
Spinal ganglia of adult rabbits were cultured in the routine and protein synthesis precursors-enriched media. On days I and 4 of cultivation, the intensity of 14C-leucine incorporation in protein and in acid soluble fraction of nerve and glial cells was determined. The tissue of the spinal ganglion keeps incorporating 14C-amino acid, into neurons and glia, for all the tested periods of cultivation with both the media employed. The curves of incorporation into the above fractions of nerve and glial cells cultured in the routine medium display similar patterns of changes, whereas those obtained from the enriched medium observations appear to be anti-fasic. The enrichment of the medium results also in less pronounced fluctuations in the intensity of the labeled amino acid in protein and 14C-leucine pool, on the tested periods of cultivation, which may provide more stable conditions of the explant's survival.
将成年兔的脊髓神经节培养于常规培养基和富含蛋白质合成前体的培养基中。在培养的第1天和第4天，测定了神经细胞和神经胶质细胞中蛋白质及酸溶性部分对¹⁴C-亮氨酸的掺入强度。在所使用的两种培养基的所有测试培养期内，脊髓神经节组织持续将¹⁴C-氨基酸掺入神经元和神经胶质细胞。在常规培养基中培养的神经细胞和神经胶质细胞上述部分的掺入曲线显示出相似的变化模式，而从富含培养基的观察结果中获得的曲线似乎呈反相。培养基的富集还导致在测试的培养期内，蛋白质中标记氨基酸的强度和¹⁴C-亮氨酸池的波动不那么明显，这可能为外植体的存活提供更稳定的条件。
