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采用两阶段聚合酶链反应-序列特异性寡核苷酸探针分型策略测定高加索人群中HLA - B等位基因的频率。

Frequency of HLA-B alleles in a Caucasoid population determined by a two-stage PCR-SSOP typing strategy.

作者信息

Middleton D, Williams F, Hamill M A, Meenagh A

机构信息

Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast, Ireland.

出版信息

Hum Immunol. 2000 Dec;61(12):1285-97. doi: 10.1016/s0198-8859(00)00186-5.

Abstract

High resolution PCR-SSOP typing methods for HLA-B identification have been established and applied to a Northern Ireland population, using large enough numbers to give dependable allele frequencies. The six systems, which operate independently of each other, are intended for use as secondary typing systems following HLA-B identification with a medium resolution PCR-SSOP technique.

摘要

已经建立了用于HLA - B鉴定的高分辨率PCR - SSOP分型方法,并将其应用于北爱尔兰人群,使用了足够多的样本数量以得出可靠的等位基因频率。这六个相互独立运行的系统,旨在作为使用中等分辨率PCR - SSOP技术进行HLA - B鉴定后的二级分型系统。

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