QUANTIPLEX HIV-1 RNA 2.0和3.0（分支DNA）检测法与AMPLICOR HIV-1 MONITOR v1.5检测法在定量血浆中人类免疫缺陷病毒1型RNA方面的比较评估

Comparative evaluation of the QUANTIPLEX HIV-1 RNA 2.0 and 3.0 (bDNA) assays and the AMPLICOR HIV-1 MONITOR v1.5 test for the quantitation of human immunodeficiency virus type 1 RNA in plasma.

作者信息

Anastassopoulou C G, Touloumi G, Katsoulidou A, Hatzitheodorou H, Pappa M, Paraskevis D, Lazanas M, Gargalianos P, Hatzakis A

机构信息

Department of Hygiene and Epidemiology, National Retrovirus Reference Center, Athens University Medical School, 75 Mikras Asias Street, GR-11527 (Goudi), Athens, Greece.

出版信息

J Virol Methods. 2001 Jan;91(1):67-74. doi: 10.1016/s0166-0934(00)00245-7.

DOI:10.1016/s0166-0934(00)00245-7

PMID:11164487

Abstract

HIV-1 RNA measurements from 84 plasma specimens obtained with the QUANTIPLEX HIV-1 RNA 2.0 and 3.0 (bDNA) assays (Chiron Diagnostics, Emeryville, CA) and with the AMPLICOR HIV-1 MONITOR Test, version 1.5 with ultra-sensitive specimen preparation (Roche Diagnostic Systems, Inc., Branchburg, NJ) were compared. The absolute RNA values of tested specimens differed significantly between bDNA 2.0 and bDNA 3.0 or Monitor v1.5 measurements (Wilcoxon signed-rank test P<0.001). Results generated with bDNA 3.0 or with Monitor v1.5 were approximately twofold greater than those generated with bDNA 2.0, with smaller differences at higher HIV-1 RNA levels and greater differences at RNA levels below 1000 copies per ml. Although highly correlated (r=0.92 and 0.86, respectively), viral load data generated with bDNA 2.0 and either bDNA 3.0 or Monitor v1.5 were in poor agreement. Concordant results (difference in log(10) copies per ml <0.5) were found at frequencies of 80% for bDNA 2.0 and bDNA 3.0 and only at 58.5% for bDNA 2.0 and Monitor v1.5. In contrast, bDNA 3.0 and Monitor v1.5 measurements were highly correlated (r=0.96) and in good agreement (92.7%).

摘要

对使用QUANTIPLEX HIV-1 RNA 2.0和3.0（分支DNA）检测法（Chiron诊断公司，加利福尼亚州埃默里维尔）以及使用AMPLICOR HIV-1监测检测法1.5版并采用超灵敏样本制备方法（罗氏诊断系统公司，新泽西州布兰奇堡）获得的84份血浆样本进行HIV-1 RNA测量，并进行比较。在分支DNA 2.0与分支DNA 3.0或监测检测法v1.5测量之间，受试样本的绝对RNA值存在显著差异（Wilcoxon符号秩检验P<0.001）。使用分支DNA 3.0或监测检测法v1.5得出的结果比使用分支DNA 2.0得出的结果大约高两倍，在较高的HIV-1 RNA水平时差异较小，而在每毫升低于1000拷贝的RNA水平时差异较大。尽管分支DNA 2.0与分支DNA 3.0或监测检测法v1.5得出的病毒载量数据高度相关（分别为r=0.92和0.86），但一致性较差。分支DNA 2.0与分支DNA 3.0的结果一致性（每毫升log(10)拷贝数差异<0.5）频率为80%，而分支DNA 2.0与监测检测法v1.5的结果一致性频率仅为58.5%。相比之下，分支DNA 3.0与监测检测法v1.5测量高度相关（r=0.96）且一致性良好（92.7%）。