Hampshire J, Altszuler N, Steele R, Greene L J
Endocrinology. 1975 Mar;96(3):822-8. doi: 10.1210/endo-96-3-822.
A sensitive radioimmunoassay is described for the measurement of plasma concentrations of canine growth hormone (cGH) as low as 0.25 ng/ml. The assay utilizes enzymatically iodinated cGH and the double antibody technique. The mean plasma concentration of growth hormone in the normal dog after overnight fast is 1.75 plus or minus .17 ng/ml. Exogenous cGH was cleared from the plasma of both the normal and hypophysectomized dog with a mean half-life of 25.6 plus or minus 1.0 min and was distributed in a volume equal to 8.9% of the body weight. Insulin hypoglycemia produced a 3- to 5-fold increase in plasma GH in 4 of 6 dogs and arginine infusion failed to produce a statistically significant rise.
本文描述了一种灵敏的放射免疫分析法，用于测量低至0.25 ng/ml的犬生长激素（cGH）血浆浓度。该分析方法采用酶促碘化cGH和双抗体技术。正常犬禁食过夜后生长激素的平均血浆浓度为1.75±0.17 ng/ml。外源性cGH从正常犬和垂体切除犬的血浆中清除，平均半衰期为25.6±1.0分钟，分布容积相当于体重的8.9%。胰岛素低血糖使6只犬中的4只血浆GH升高3至5倍，而精氨酸输注未能产生统计学上的显著升高。
