Yamashita M, Iwabuchi K
Tokyo University of Agriculture and Technology, Saiwai-cho, Fuchu, 183-8509, Tokyo, Japan
J Insect Physiol. 2001 Apr;47(4-5):325-31. doi: 10.1016/s0022-1910(00)00144-x.
We followed the fate of microcultured Bombyx mori prohemocytes in vitro. Prohemocytes isolated from larval hemolymph (day 1 of 4th instar) were maintained for 4-11 days in serum-free MGM-450 medium and some of them underwent mitotic division. Over 60% of the non-dividing prohemocytes differentiated to plasmatocytes or granulocytes. Some of the granulocytes subsequently transformed to spherulocytes. Of the dividing prohemocytes, 59.2% of the daughter cells differentiated into other types of hemocytes such as plasmatocytes, granulocytes and spherulocytes, and the remainder divided into new prohemocytes. Four of these renewed prohemocytes generated daughter cells composed of plasmatocytes and granulocytes. These results suggest that prohemocytes possess the properties of stem cells, and that plasmatocytes and spherulocytes may be terminally differentiated cells, whereas granulocytes, at least in part, may be a transient form of spherulocyte. Oenocytoids were not produced, suggesting that the lineage of oenocytoids differs from that of other types of hemocytes and that it is determined before release from hemopoietic organs.
我们在体外追踪了微培养的家蚕原血细胞的命运。从幼虫血淋巴（第4龄第1天）中分离出的原血细胞在无血清的MGM - 450培养基中培养4 - 11天，其中一些进行了有丝分裂。超过60%未分裂的原血细胞分化为浆细胞或粒细胞。一些粒细胞随后转变为球血细胞。在进行分裂的原血细胞中，59.2%的子细胞分化为其他类型的血细胞，如浆细胞、粒细胞和球血细胞，其余的则分裂为新的原血细胞。这些新产生的原血细胞中有4个产生了由浆细胞和粒细胞组成的子细胞。这些结果表明原血细胞具有干细胞的特性，浆细胞和球血细胞可能是终末分化细胞，而粒细胞至少部分可能是球血细胞的一种过渡形式。未产生oenocytoids，这表明oenocytoids的谱系与其他类型的血细胞不同，并且在从造血器官释放之前就已确定。
