Vengateswari Govindaraj, Arunthirumeni Murugan, Shivakumar Muthugounder Subramanian
Molecular Entomology Laboratory, Department of Biotechnology, Periyar University, Salem-11, Tamil Nadu, India.
Toxicol Rep. 2020 Oct 8;7:1428-1437. doi: 10.1016/j.toxrep.2020.10.005. eCollection 2020.
The larvae of (Fabricius) were reared on five host plants, , , , , and . The larvae were immunized with to observe the immune response. The results of total and differential hemocyte count were increased in , , and fed larval hemolymph. Similar results were observed in the parameter of nodulation, melanization, and phenoloxidase. Total protein was higher in fed larvae. Antioxidant levels like Catalase (CAT), Superoxide dismutase (SOD), Glutathione S- transferase (GST), Peroxidase (POX), Lipid peroxidase (LPO), and Esterase (EST) was found in moreover all plant-feeding insect. High CAT activity was observed 2-6 h in , , and fed larval midgut and fatbody samples. Increased SOD activity in both midgut and fatbody at 2-12 h of , , and fed. GST activity was increased initially 2-6 h in and . Increased POX activity was observed initially in all treated groups. Highest LPO observed at 6 h in in both midgut and fatbody. Whereas increased EST activity was observed in and . The results of the present study shows that nature of food influence the immunity against Bt infection. This information can be very useful for incorporating biological control program for insect pest.
（法布里丘斯）的幼虫在五种寄主植物上饲养，分别是 、 、 、 和 。用 对幼虫进行免疫以观察免疫反应。在喂食 幼虫血淋巴的 、 和 中，血细胞总数和分类计数结果增加。在结瘤、黑化和酚氧化酶参数方面也观察到类似结果。喂食 的幼虫总蛋白含量更高。在所有以植物为食的昆虫中都发现了抗氧化剂水平，如过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽 - S - 转移酶（GST）、过氧化物酶（POX）、脂质过氧化物酶（LPO）和酯酶（EST）。在喂食 的 、 和 的幼虫中肠和脂肪体样本中，2 - 6小时观察到高CAT活性。在喂食 的 、 和 中，中肠和脂肪体在2 - 12小时时SOD活性增加。在 和 中，GST活性在最初2 - 6小时增加。在所有处理组中最初都观察到POX活性增加。在 中肠和脂肪体中，6小时时观察到最高的LPO。而在 和 中观察到EST活性增加。本研究结果表明食物性质会影响对Bt感染的免疫力。该信息对于纳入害虫生物防治计划可能非常有用。
