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cDNA detection and analysis.

作者信息

Bashiardes S, Lovett M

机构信息

Department of Genetics, Washington University School of Medicine, Campus Box 8232, 4566 Scott Avenue, St Louis, Missouri 63110-1093, USA.

出版信息

Curr Opin Chem Biol. 2001 Feb;5(1):15-20. doi: 10.1016/s1367-5931(00)00161-7.

Abstract

In the continuing search for a full-length cDNA cloning method, there is no clear winner. Perfecting these techniques may require the re-engineering of reverse transcriptase. There now exist two reasonably linear methods for deriving expression signatures from small amounts of biological material, but advances in serial analysis of gene expression provide a quantitative, if expensive, alternative to these methods.

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