日本脑炎病毒非结构蛋白NS1抗体：基于单克隆抗体的间接酶联免疫吸附测定在区分感染与疫苗接种方面的潜在应用
Antibody to the nonstructural protein NS1 of Japanese encephalitis virus: potential application of mAb-based indirect ELISA to differentiate infection from vaccination.
作者信息
Shu P Y, Chen L K, Chang S F, Yueh Y Y, Chow L, Chien L J, Chin C, Lin T H, Huang J H
机构信息
Division of Vector-borne Infectious Diseases, Center for Disease Control, Department of Health, 161 Kun-Yang Street, Taipei, Taiwan, PR China.
出版信息
Vaccine. 2001 Feb 8;19(13-14):1753-63. doi: 10.1016/s0264-410x(00)00391-1.
An indirect enzyme-linked immunosorbent assay (ELISA) was developed to detect and differentiate the antibody responses to Japanese encephalitis (JE) virus nonstructural protein NS1 between infected and vaccinated individuals. The results showed that all convalescent sera from JE patients contained NS1-specific IgG antibodies, while 65 and 40% of these sera showed detectable NS1-specific IgM and IgA antibodies, respectively. Specificity analysis showed that NS1-specific IgM and IgA antibodies from JE patients do not cross-react to dengue virus NS1 glycoprotein, while IgG antibodies from 10% of JE patients showed significant cross-reaction to dengue virus NS1 glycoprotein. To differentiate infection from vaccination, the immune sera from 24 children vaccinated with inactivated JE vaccine were analyzed. The data showed that none of these immune sera had detectable NS1-specific IgG antibodies. The results demonstrated the potential application of JE NS1-specific indirect ELISA to differentiate infection from vaccination.
开发了一种间接酶联免疫吸附测定（ELISA），以检测和区分感染个体与接种疫苗个体对日本脑炎（JE）病毒非结构蛋白NS1的抗体反应。结果显示，所有JE患者的恢复期血清均含有NS1特异性IgG抗体，而这些血清中分别有65%和40%显示可检测到NS1特异性IgM和IgA抗体。特异性分析表明，JE患者的NS1特异性IgM和IgA抗体与登革病毒NS1糖蛋白无交叉反应，而10%的JE患者的IgG抗体与登革病毒NS1糖蛋白有显著交叉反应。为了区分感染与疫苗接种，分析了24名接种灭活JE疫苗儿童的免疫血清。数据显示，这些免疫血清中均未检测到NS1特异性IgG抗体。结果证明了JE NS1特异性间接ELISA在区分感染与疫苗接种方面的潜在应用。
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