用于区分登革病毒原发性和继发性感染的捕获免疫球蛋白M（IgM）和IgG酶联免疫吸附测定（ELISA）以及非结构蛋白NS1血清型特异性IgG ELISA的比较
Comparison of capture immunoglobulin M (IgM) and IgG enzyme-linked immunosorbent assay (ELISA) and nonstructural protein NS1 serotype-specific IgG ELISA for differentiation of primary and secondary dengue virus infections.
作者信息
Shu Pei-Yun, Chen Li-Kuang, Chang Shu-Fen, Yueh Yi-Yun, Chow Ling, Chien Li-Jung, Chin Chuan, Lin Ting-Hsiang, Huang Jyh-Hsiung
机构信息
Division of Research Development and Laboratory Diagnosis, Center for Disease Control, Department of Health, Taipei, Taiwan, Republic of China.
出版信息
Clin Diagn Lab Immunol. 2003 Jul;10(4):622-30. doi: 10.1128/cdli.10.4.622-630.2003.
Abstract
We have found that NS1 serotype-specific immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) can be used to differentiate primary and secondary dengue virus infections. This is due to the fact that the NS1-specific IgG antibody cannot be detected before day 9 of illness for primary infection, so the NS1-specific IgG antibodies measured in acute-phase sera must come from previous infection. Comparison of NS1 serotype-specific IgG ELISA with envelope- and membrane-specific capture IgM and IgG ELISA in the differentiation of primary and secondary dengue virus infections showed good correlation (95.90% agreement). Most important, we have found that the serotype of the dengue virus from the majority of patients with primary infection could be correctly identified when convalescent-phase or postinfection sera were analyzed by NS1 serotype-specific IgG ELISA. These findings suggested that NS1 serotype-specific IgG ELISA could be reliably applied for serodiagnosis and seroepidemiological study of dengue virus infection.
摘要
我们发现，NS1血清型特异性免疫球蛋白G（IgG）酶联免疫吸附测定（ELISA）可用于区分登革病毒的初次感染和二次感染。这是因为对于初次感染，在发病第9天之前无法检测到NS1特异性IgG抗体，所以急性期血清中检测到的NS1特异性IgG抗体必定来自既往感染。在区分登革病毒初次感染和二次感染方面，将NS1血清型特异性IgG ELISA与包膜和膜特异性捕获IgM及IgG ELISA进行比较，结果显示具有良好的相关性（一致性为95.90%）。最重要的是，我们发现，当通过NS1血清型特异性IgG ELISA分析恢复期或感染后血清时，大多数初次感染患者的登革病毒血清型能够被正确识别。这些发现表明，NS1血清型特异性IgG ELISA可可靠地应用于登革病毒感染的血清学诊断和血清流行病学研究。
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