Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences, Ekaterinburg, Russia.
Bull Exp Biol Med. 2024 Oct;177(6):721-724. doi: 10.1007/s10517-024-06257-y. Epub 2024 Oct 23.
Under the influence of inflammation, pancreatic β cells can transdifferentiate into cells with a different phenotype. When inflammation decreases, the opposite process is possible. We studied the effect of intramuscular injection of 5-amino-2,3-dihydrophthalazine-1,4-dione sodium salt (APH) on the structural and functional characteristics of the pancreatic islets in rats with experimental type 2 diabetes mellitus. Insulin-producing, glucagon-producing, and proliferating cells were identified by immunohistochemistry. After APH administration, an increase in the number of β cells, a decrease in the number of α cells and cells synthesizing both insulin and glucagon (insulin-glucagon-positive) were observed; mitotic activity of β cells did not change. It is likely that APH promotes transdifferentiation of α cells into β cells by changing the microenvironment of endocrine cells and reducing inflammation in pancreatic islets.
在炎症的影响下，胰腺β细胞可以转分化为具有不同表型的细胞。当炎症减轻时，可能会发生相反的过程。我们研究了肌内注射 5-氨基-2,3-二氢酞嗪-1,4-二酮钠盐（APH）对实验性 2 型糖尿病大鼠胰岛结构和功能特征的影响。通过免疫组织化学鉴定胰岛素产生细胞、胰高血糖素产生细胞和增殖细胞。在给予 APH 后，观察到β细胞数量增加，α细胞和同时合成胰岛素和胰高血糖素的细胞（胰岛素-胰高血糖素阳性）数量减少；β细胞的有丝分裂活性没有改变。APH 可能通过改变内分泌细胞的微环境和减少胰岛炎症来促进α细胞向β细胞的转分化。
