Eichelbaum M, Bertilsson L
J Chromatogr. 1975 Jan 14;103(1):135-40. doi: 10.1016/s0021-9673(00)83807-8.
A liquid-chromatographic method for the simultaneous determination of carbamazepine and its active metabolite (carbamazepine 10,11-epoxide) in plasma has been developed. The two compounds were identified in plasma by mass spectrometry. The lower limit of sensitivity is about 4 and 40 ng for the drug and its metabolite, respectively. 10,11-Dihydrocarbamazepine is used as internal standard for the determinations, which have precisions of 2.2 and 4.2%, respectively. No derivatization is needed. The specificity of the method for carbamazepine is shown by the significant correlation (r = 0.99) between the results obtained by this method and by mass fragmentography for the drug in plasma of patients.
已开发出一种液相色谱法，用于同时测定血浆中的卡马西平及其活性代谢物（卡马西平10,11 - 环氧化物）。通过质谱法在血浆中鉴定出这两种化合物。该药物及其代谢物的灵敏度下限分别约为4纳克和40纳克。10,11 - 二氢卡马西平用作测定的内标，测定的精密度分别为2.2%和4.2%。无需衍生化。该方法对卡马西平的特异性通过该方法与患者血浆中药物的质量碎片分析法所得结果之间的显著相关性（r = 0.99）得以体现。
