蛋白质在可控孔径玻璃柱上色谱分析中的离子效应。

Ionic effects in the chromatography of proteins on columns of controlled-pore glass.

作者信息

Crone H D, Dawson R M, Smith E M

出版信息

J Chromatogr. 1975 Jan 14;103(1):71-5. doi: 10.1016/s0021-9673(00)83802-9.

Abstract

Bovine serum albumin, acetylcholinesterase and two non-protein markers were chromatographed on columns of controlled-pore glasses in buffer solutions of varied ionic strengths. Adsorption of protein to the glass and ionic effects due to repulsion of the negatively-charged solutes from the glass matrix were observed. When glasses were treated with Carbowax 20M, the adsorption disappeared but ion exclusion effects persisted. The ionic behavior of Carbowax-treated glasses was similar to that observed by one of the authors when using agarose columns.

摘要

牛血清白蛋白、乙酰胆碱酯酶和两种非蛋白质标记物在不同离子强度的缓冲溶液中于可控孔径玻璃柱上进行色谱分析。观察到蛋白质在玻璃上的吸附以及由于带负电荷的溶质从玻璃基质排斥而产生的离子效应。当玻璃用聚乙二醇20M处理时，吸附消失，但离子排斥效应仍然存在。聚乙二醇处理过的玻璃的离子行为与其中一位作者使用琼脂糖柱时观察到的相似。