Mizutani T, Mizutani A
J Chromatogr. 1979 Jan 11;168(1):143-50. doi: 10.1016/s0021-9673(00)80703-7.
As controlled-pore glass (CPG) has anionic silanol groups, we compared the elution patterns of proteins chromatographed on CPG with those on carboxymethyl (CM)-cellulose using standard proteins and two protein mixtures, rabbit serum and bovine parotid extracts. The results showed that the order of proteins eluted from CPG was similar to that from CM-cellulose, although some differences were found. The adsorption of proteins on CPG was slightly stronger than that on CM-cellulose and the separation of proteins on CPG was slightly better. Some conditions, such as amounts of proteins loaded on the column, pH, temperature and kind of buffer solution for elution, were investigated. The pH of buffer solutions was important in the adsorption chromatography of proteins on CPG and the useful pH range was 7.0-8.5.
由于可控孔径玻璃（CPG）含有阴离子硅醇基团，我们使用标准蛋白质以及两种蛋白质混合物（兔血清和牛腮腺提取物），比较了在CPG上进行色谱分离的蛋白质与在羧甲基（CM）纤维素上的蛋白质洗脱模式。结果表明，从CPG上洗脱的蛋白质顺序与从CM纤维素上洗脱的顺序相似，尽管存在一些差异。蛋白质在CPG上的吸附略强于在CM纤维素上的吸附，并且蛋白质在CPG上的分离效果略好。研究了一些条件，如柱上加载的蛋白质量、pH值、温度和洗脱缓冲液的种类。缓冲溶液的pH值在蛋白质在CPG上的吸附色谱中很重要，有用的pH范围是7.0 - 8.5。
