Hill J H, Wainer B H, Fitch F W, Rothberg R M
J Immunol. 1975 Apr;114(4):1363-8.
Morphine clearance from the circulation of normal rabbits and rabbits with circulating anti-morphine antibody was studied. Individual animals were injected with 14C trace-labeled morphine in saline (6 mg morphine/kg body weight). The rabbits were bled at various times after morphine injection and the amount of morphine present in the serum at each time interval was determined. Morphine could be detected in the serum of normal animals for 1 week following injection and up to 12 weeks following injection in at least one animal with antibodies to morphine. The rate of morphine clearance in animals immunized to morphine-6-hemisuccinate-bovine serum albumin was not significantly different from normal during the first 4 hr after morphine injection. However, by 24 hr after injection, the rate of morphine clearance in morphine-6-hemisuccinate-bovine serum albumin-immunized animals was significantly slower than in normal animals. The amount of morphine present in the sera of the rabbits 24 hr after injection was related to the antigen-binding capacity and independent of antibody avidity. However, the rate of morphine clearance at times greater than 24 hr after injection was related to the average antibody avidity and not related to the antigen-binding capacity.
研究了正常兔子和体内存在循环抗吗啡抗体的兔子体内吗啡从循环系统中的清除情况。给每只动物静脉注射溶于生理盐水的14C示踪标记吗啡（6毫克吗啡/千克体重）。在注射吗啡后的不同时间点采集兔子的血液样本，测定每个时间间隔血清中吗啡的含量。正常动物注射后1周内血清中均可检测到吗啡，至少有一只体内存在吗啡抗体的动物在注射后12周仍可检测到吗啡。在注射吗啡后的前4小时内，对吗啡-6-半琥珀酸-牛血清白蛋白免疫的动物体内吗啡的清除率与正常动物无显著差异。然而，注射后24小时，对吗啡-6-半琥珀酸-牛血清白蛋白免疫的动物体内吗啡的清除率明显低于正常动物。注射后24小时兔子血清中吗啡的含量与抗原结合能力有关，与抗体亲和力无关。然而，注射后超过24小时的吗啡清除率与平均抗体亲和力有关，与抗原结合能力无关。
