O'Neill S P, Robb T E
J Immunol. 1976 Feb;116(2):363-6.
Animals immunized with hapten-protein conjugates subsequently circulate high concentrations of hapten bound by antibody. The levels of hapten detected are capable of significantly reducing antibody titer in the sera immunized animals. In the case of steroid-protein conjugates, the main source of increased plasma steroid concentration is the immunizing conjugate, although a contribution from increased host secretion may also occur. The results for rabbits immunized with digoxin-BSA indicate that the appearance of circulating digoxin followed the appearance of circulating antibody to digoxin. Appearance of digoxin in circulation appears to coincide with the operation of the immune response and may be related to macrophage activity. Similar conclusions are drawn from results obtained for circulating morphine in the serum of a sheep immunized with morphine-BSA. Injected hapten-protein antigens are probably processed by macrophage to produce low molecular weight haptenic fragments which are maintained in circulation for prolonged periods in the form of antibody-hapten complexes.
用半抗原-蛋白质偶联物免疫的动物随后会循环出高浓度的与抗体结合的半抗原。检测到的半抗原水平能够显著降低免疫动物血清中的抗体滴度。就类固醇-蛋白质偶联物而言，血浆类固醇浓度升高的主要来源是免疫偶联物，不过宿主分泌增加也可能有一定作用。用洋地黄毒苷-牛血清白蛋白免疫兔子的结果表明，循环中洋地黄毒苷的出现紧随循环中抗洋地黄毒苷抗体的出现。循环中洋地黄毒苷的出现似乎与免疫反应的发生同时出现，并且可能与巨噬细胞活性有关。从用吗啡-牛血清白蛋白免疫的绵羊血清中循环吗啡的结果也得出了类似结论。注射的半抗原-蛋白质抗原可能被巨噬细胞处理以产生低分子量的半抗原片段，这些片段以抗体-半抗原复合物的形式在循环中长时间维持。
