Thomas Patricia A, Oykutlu Dilek, Pou Bel, Tyler Denise, Oberley Larry W, Robinson Robert A, Lenel Julia C
University of lowa Hospitals and Clinics, Department of Pathology and the Radiation Research Laboratory, Iowa, USA.
Pathol Oncol Res. 1997;3(4):278-286. doi: 10.1007/BF02904287.
Intrinsic antioxidant enzymes (AE) are essential for protection against potential cellular damage by free radicals (FRs), which affect a variety of biological processes. The levels or activities of AEs can be abnormal in human malignancies in general, and FR production is a possible mechanism of estrogen related carcinogenesis specifically. However, the role of AEs in breast cancer ramains unclear. Immunodetectable AEs were characterized in 95 node negative cancers using rabbit polyclonal antibodies. Results were correlated with established and experimental biomarkers of breast cancer. AEs were greater than benign differentiated epithelium in more than 40% and lower in 10-14% of tumors. Patterns of staining were enzyme and tumor pattern specific. Increased immunodetectable AE was associated with large, poorly differentiated tumors, and younger age. Catalase correlated with nuclear grade and disease related death (p< 0.05), and highlighted tumor microvasculature. Additional work in this area may further elucidate the role of AEs in breast cancer growth and progression.
内源性抗氧化酶（AE）对于保护细胞免受自由基（FR）潜在的损伤至关重要，自由基会影响多种生物学过程。一般来说，在人类恶性肿瘤中，AE的水平或活性可能异常，特别是FR的产生是雌激素相关致癌作用的一种可能机制。然而，AE在乳腺癌中的作用仍不清楚。使用兔多克隆抗体对95例淋巴结阴性癌症中的免疫可检测AE进行了表征。结果与已确立的和实验性的乳腺癌生物标志物相关。在超过40%的肿瘤中，AE高于良性分化上皮，在10%-14%的肿瘤中较低。染色模式具有酶和肿瘤特异性。免疫可检测AE增加与肿瘤体积大、分化差以及患者年龄较轻有关。过氧化氢酶与核分级和疾病相关死亡相关（p<0.05），并突出显示肿瘤微血管。该领域的进一步研究可能会进一步阐明AE在乳腺癌生长和进展中的作用。
