Rothman F G, Robbins A R, Lackey D
J Virol. 1975 Apr;15(4):1024-8. doi: 10.1128/JVI.15.4.1024-1028.1975.
The properties of two rII complementation heterozygotes (D5B and D7A) of bacteriophage T4 are described. These strains are characterized by their stability, each forming less than 10-3 r segregants among their viable progeny, and by their segregation of only one of the two parental types. No increase in r progeny was found on crossing D7A or D5B with T4r+, indicating that the duplications in these strains are not separated by an essential region of the phage genome. Both D5B and D7A from h-2+/h-4+ heterozygotes at frequencies similar to T4r+, suggesting that the duplicated regions in these strains are short. The progeny of these h-2+/h-4+ heterozygotes retain heterozygosity for rII but not for h: therefore, D5B and D7A are not stabilized terminal redundancy complementation heterozygotes. We conclude that D5B and D7A contain very short tandem duplications and we present structures consistent with the observed characteristics of these phages.
描述了噬菌体T4的两种rII互补杂合子（D5B和D7A）的特性。这些菌株的特点是具有稳定性，在其存活后代中，每种菌株形成的r分离株少于10^-3，并且仅分离出两种亲本类型中的一种。将D7A或D5B与T4r+杂交时，未发现r后代增加，这表明这些菌株中的重复序列没有被噬菌体基因组的必需区域隔开。D5B和D7A都以与T4r+相似的频率从h-2+/h-4+杂合子中产生，这表明这些菌株中的重复区域很短。这些h-2+/h-4+杂合子的后代对rII保持杂合性，但对h不保持杂合性：因此，D5B和D7A不是稳定的末端冗余互补杂合子。我们得出结论，D5B和D7A包含非常短的串联重复序列，并且我们提出了与这些噬菌体观察到的特征一致的结构。
