McAlinden A, Dudhia J, Bolton M C, Lorenzo P, Heinegård D, Bayliss M T
Department of Orthopaedic Surgery, Washington University School of Medicine, St Louis, Missouri 63110, USA.
Osteoarthritis Cartilage. 2001 Jan;9(1):33-41. doi: 10.1053/joca.2000.0347.
To determine if the biosynthesis of aggrecan and decorin in the human meniscus and the potential of the cells to express these macromolecules (mRNA), is affected by the age of the individual and that if any changes are observed are they different to those measured in articular cartilage obtained from the same joint.
Radiolabelling of tissue explants, anion-exchange chromatography and agarose-polyacrylamide gel electrophoresis were used to analyze newly synthesized proteoglycans. A quantitative, competitive reverse-transcriptase polymerase chain reaction was developed and applied to the tissue to measure the expression of decorin and aggrecan mRNA.
Proteoglycan synthesis in the meniscus was higher in young donors (1-5 mmoles sulfate incorporated/h/mgDNA, under 20 years of age) than in adult tissues (0.5-1 mmoles incorporated/h/mgDNA, 20-62 years of age) and decorin was the major proteoglycan synthesized at this time. An age-related increase in the proportion of aggrecan synthesis in the meniscus was also observed using agarose-polyacrylamide gel electrophoresis. Both decorin (five-fold) and aggrecan (eight-fold) mRNA expression increased with age in meniscus whereas levels were relatively constant in articular cartilage. In addition, the synthesis of decorin and aggrecan and the expression of their mRNA was different in meniscus and articular cartilage from the same knee joint.
The synthesis and turnover of aggrecan and decorin in the human meniscus is influenced by the age of the individual and is not the same as that observed for articular cartilage.
确定人半月板中聚集蛋白聚糖和核心蛋白聚糖的生物合成以及细胞表达这些大分子（mRNA）的潜力是否受个体年龄影响，以及如果观察到任何变化，这些变化是否与从同一关节获取的关节软骨中测量到的变化不同。
采用组织外植体放射性标记、阴离子交换色谱和琼脂糖 - 聚丙烯酰胺凝胶电泳分析新合成的蛋白聚糖。开发了一种定量竞争性逆转录聚合酶链反应并应用于组织以测量核心蛋白聚糖和聚集蛋白聚糖mRNA的表达。
半月板中蛋白聚糖的合成在年轻供体（年龄在20岁以下，每小时每毫克DNA掺入1 - 5毫摩尔硫酸盐）中高于成人组织（年龄在20 - 62岁，每小时每毫克DNA掺入0.5 - 1毫摩尔），此时核心蛋白聚糖是主要合成的蛋白聚糖。使用琼脂糖 - 聚丙烯酰胺凝胶电泳还观察到半月板中聚集蛋白聚糖合成比例随年龄增加。半月板中核心蛋白聚糖（五倍）和聚集蛋白聚糖（八倍）的mRNA表达均随年龄增加，而在关节软骨中水平相对恒定。此外，同一膝关节的半月板和关节软骨中核心蛋白聚糖和聚集蛋白聚糖的合成及其mRNA表达不同。
人半月板中聚集蛋白聚糖和核心蛋白聚糖的合成与周转受个体年龄影响，且与关节软骨中观察到的情况不同。
