Vranová E, Tähtiharju S, Sriprang R, Willekens H, Heino P, Palva E T, Inzé D, Van Camp W
Vakgroep Moleculaire Genetica and Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
J Exp Bot. 2001 Jan;52(354):181-2.
The AKT3 potassium channel protein was identified as a strongly interacting partner of the Arabidopsis thaliana protein phosphatase 2C (AtPP2CA) in a yeast two-hybrid screen. A deletion analysis indicated that the catalytic domain of AtPP2CA was essential for the interaction with AKT3. Furthermore, the related PP2C phosphatase ABI1 did not interact with AKT3 in yeast.
在酵母双杂交筛选中,AKT3钾通道蛋白被鉴定为拟南芥蛋白磷酸酶2C(AtPP2CA)的强相互作用伴侣。缺失分析表明,AtPP2CA的催化结构域对于与AKT3的相互作用至关重要。此外,相关的PP2C磷酸酶ABI1在酵母中不与AKT3相互作用。