Gene Discovery Research Group, RIKEN Plant Science Center, 3-1-1 Kouyadai, Tsukuba, Ibaraki 305-0074, Japan.
J Plant Res. 2011 Jul;124(4):539-48. doi: 10.1007/s10265-011-0418-x. Epub 2011 Apr 2.
Recent advances in our understanding of abscisic acid (ABA) signaling have identified a core pathway consisting of receptors (PYR/PYL/RCAR), protein phosphatases (PP2C), protein kinases (SnRK2), and several downstream factors that will lead to the next stage of ABA research. Systems biology will be an important concept for further understanding ABA responses in plants. In this review, two practical approaches of systems biology to ABA signaling are presented: the one is 'transcriptome analysis', which covers coding genes as well as unannotated transcripts, and the other is 'phosphoproteomics'. The latter technology will offer an unprecedented overview of the regulatory networks involved in ABA signaling because protein phosphorylation/dephosphorylation is a major center of such regulation. Systematic studies will contribute to our understanding of the network structure and dynamics of ABA signaling; moreover, systems biology will facilitate ABA signaling studies as well as future biotechnological applications in crops or trees.
近年来，我们对脱落酸（ABA）信号转导的理解取得了一些进展，鉴定出一个由受体（PYR/PYL/RCAR）、蛋白磷酸酶（PP2C）、蛋白激酶（SnRK2）和几个下游因子组成的核心途径，这些因子将引领 ABA 研究的下一阶段。系统生物学将是进一步理解植物中 ABA 响应的一个重要概念。在这篇综述中，介绍了两种用于 ABA 信号转导的系统生物学的实用方法：一种是“转录组分析”，它涵盖了编码基因和未注释的转录本，另一种是“磷酸化蛋白质组学”。后一种技术将提供一个前所未有的涉及 ABA 信号转导的调控网络概览，因为蛋白质磷酸化/去磷酸化是这种调控的主要中心。系统研究将有助于我们理解 ABA 信号转导的网络结构和动态；此外，系统生物学将促进 ABA 信号转导的研究以及未来在作物或树木中的生物技术应用。
