Natalucci S, Ruggeri P, Cogo C E, Picchio V, Burattini R
Department of Electronics and Automatics, University of Ancona, Italy.
Exp Physiol. 2000 Nov;85(6):775-81. doi: 10.1017/s0958067000020637.
This study was performed to compare glucose metabolism in anaesthetised spontaneously hypertensive rats (SHR) and Wistar Kyoto rats (WKY) in an attempt to clarify whether this animal model of hypertension approximates the insulin-resistant state seen in human hypertension. With this aim the minimal model of glucose kinetics was applied to glucose and insulin data derived from a 12-sample, 120 min intravenous glucose tolerance test (IVGTT) performed in ten SHR and nine WKY rats under pentobarbital anaesthesia. This method provided two metabolic indices: the glucose effectiveness, S(G), which quantifies the ability of glucose per se to enhance its rate of disappearance and to inhibit hepatic glucose production, and the insulin sensitivity, S(I), which measures the ability of insulin to enhance plasma glucose disappearance and to inhibit hepatic glucose production. Systolic and diastolic arterial pressures in the SHR group were significantly higher (P < 0.0005) than in the WKY group. Mean S(G) and S(I) estimates from the SHR group (S(G) = 16.2 (+/- 2.0) x 10(-2) dl x min(-1) x kg(-1) and S(I) = 12.5 (+/- 1.9) x 10(-4) dl x min(-1) x kg(-1) (microU ml(-1))(-1)) were not significantly different (P > 0.05) from mean estimates that characterised the WKY group (S(G) = 13.1 (+/- 1.5) x 10(-2) dl x min(-1) x kg(-1) and S(I) = 15.8 (+/- 4.3) x 10(-4) dl x min(-1) x kg(-1) (microU ml(-1))(-1)). This result is in contrast with reported findings from humans in which insulin sensitivity is significantly reduced in the presence of hypertension.
本研究旨在比较麻醉状态下的自发性高血压大鼠（SHR）和Wistar Kyoto大鼠（WKY）的葡萄糖代谢情况，以阐明这种高血压动物模型是否类似于人类高血压中所见的胰岛素抵抗状态。为此，将葡萄糖动力学的最小模型应用于戊巴比妥麻醉下对10只SHR和9只WKY大鼠进行的12样本、120分钟静脉葡萄糖耐量试验（IVGTT）所获得的葡萄糖和胰岛素数据。该方法提供了两个代谢指标：葡萄糖效能S(G)，它量化葡萄糖本身提高其消失速率和抑制肝葡萄糖生成的能力；以及胰岛素敏感性S(I)，它衡量胰岛素提高血浆葡萄糖消失速率和抑制肝葡萄糖生成的能力。SHR组的收缩压和舒张压显著高于WKY组（P < 0.0005）。SHR组的平均S(G)和S(I)估计值（S(G) = 16.2（±2.0）×10⁻² dl×min⁻¹×kg⁻¹，S(I) = 12.5（±1.9）×10⁻⁴ dl×min⁻¹×kg⁻¹（μU ml⁻¹）⁻¹）与表征WKY组的平均估计值（S(G) = 13.1（±1.5）×10⁻² dl×min⁻¹×kg⁻¹，S(I) = 15.8（±4.3）×10⁻⁴ dl×min⁻¹×kg⁻¹（μU ml⁻¹）⁻¹）相比，差异无统计学意义（P > 0.05）。这一结果与人类的报道结果相反，在人类中，高血压存在时胰岛素敏感性会显著降低。
