荧光假单胞菌ATCC 17400的海藻糖操纵子。

The trehalose operon of Pseudomonas fluorescens ATCC 17400.

作者信息

Matthijs S, Koedam N, Cornelis P, De Greve H

机构信息

Laboratorium Microbiële Interacties, Vrije Universiteit Brussel, Sint-Genesius-Rode, Belgium.

出版信息

Res Microbiol. 2000 Dec;151(10):845-51. doi: 10.1016/s0923-2508(00)01151-7.

DOI:10.1016/s0923-2508(00)01151-7

PMID:11191810

Abstract

The trehalose operon of Pseudomonas fluorescens ATCC 17400 consists of treP, treA and treR. The gene treP codes for a putative enzyme II subunit of the phosphotransferase system that catalyzes the phosphorylation of trehalose together with its translocation across the cell membrane and treA encodes a putative phosphotrehalase, which hydrolyzes the incoming trehalose-6-phosphate into glucose and glucose-6-phosphate. Both genes are negatively regulated by TreR, a repressor of the FadR-GntR family of transcription regulators. The operon that is induced by trehalose present in the medium shows a high similarity both in the function of genes and in the regulation with the trehalose operon of Bacillus subtilis.

摘要

荧光假单胞菌ATCC 17400的海藻糖操纵子由treP、treA和treR组成。基因treP编码磷酸转移酶系统的一个假定的酶II亚基，该亚基催化海藻糖的磷酸化及其跨细胞膜的转运，而treA编码一种假定的磷酸海藻糖酶，它将进入的6-磷酸海藻糖水解为葡萄糖和6-磷酸葡萄糖。这两个基因均受TreR负调控，TreR是转录调节因子FadR-GntR家族的一种阻遏物。该操纵子由培养基中存在的海藻糖诱导，在基因功能和调控方面与枯草芽孢杆菌的海藻糖操纵子具有高度相似性。