对枯草芽孢杆菌treA基因侧翼DNA的分析揭示了编码一种假定的特异性酶IITre和海藻糖操纵子潜在调节因子的基因。

Analysis of DNA flanking the treA gene of Bacillus subtilis reveals genes encoding a putative specific enzyme IITre and a potential regulator of the trehalose operon.

作者信息

Schöck F, Dahl M K

机构信息

Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Germany.

出版信息

Gene. 1996 Oct 10;175(1-2):59-63. doi: 10.1016/0378-1119(96)00120-5.

DOI:10.1016/0378-1119(96)00120-5

PMID:8917076

Abstract

Nucleotide sequencing revealed the genes treP encoding a putative specific enzyme IITre upstream from treA and treR encoding a potential regulator downstream from treA of Bacillus subtilis 168. The treP gene encodes a 470-amino acid (aa) protein (50 kDa) showing high similarities to several different specific enzymes II of phosphoenolpyruvate-dependent phosphotransferase systems. treR encodes a 238-aa protein (28 kDa) with high homologies in its N-terminal part to DNA-binding proteins including a helixturn-helix motif. Homologies in its C-terminal part place it in the family of FadR-GntR transcriptional regulators. The three genes, treP-treA-treR, are probably organized in one operon expressed by a sigma A-dependent promoter 53 bp upstream from treP and a rho-independent terminator 28 bp downstream from treR.

摘要

核苷酸测序显示，在枯草芽孢杆菌168的treA上游存在编码假定特异性酶IITre的treP基因，在treA下游存在编码潜在调控因子的treR基因。treP基因编码一个470个氨基酸（aa）的蛋白质（50 kDa），与磷酸烯醇丙酮酸依赖性磷酸转移酶系统的几种不同特异性酶II具有高度相似性。treR编码一个238个氨基酸（28 kDa）的蛋白质，其N端部分与包括螺旋-转角-螺旋基序在内的DNA结合蛋白具有高度同源性。其C端部分的同源性使其属于FadR-GntR转录调节因子家族。treP-treA-treR这三个基因可能由一个操纵子组成，该操纵子由treP上游53 bp处的σA依赖性启动子和treR下游28 bp处的ρ非依赖性终止子表达。