枯草芽孢杆菌168的tre操纵子的表达受阻遏蛋白TreR的调控。
Expression of the tre operon of Bacillus subtilis 168 is regulated by the repressor TreR.
作者信息
Schöck F, Dahl M K
机构信息
Lehrstuhl für Mikrobiologie, Institut fur Mikrobiologie, Biochemie undGenetik der Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
出版信息
J Bacteriol. 1996 Aug;178(15):4576-81. doi: 10.1128/jb.178.15.4576-4581.1996.
Abstract
The tre locus from Bacillus subtilis containing the genes treP, treA, and treR has been analyzed for its regulation. We demonstrate that at least treP and treA form an operon whose expression is regulated at the transcriptional level. TreR activity has been investigated in in vivo and in vitro studies. An insertional inactivation of treR led to a constitutive expression of treP and treA. Upstream of treP we identified a 248-bp DNA fragment containing a potential sigmaA-dependent promoter and two palindromes reflecting potential tre operators which led to complex formation with TreR-containing protein extracts in DNA retardation experiments. This complex formation is abolished in the presence of trehalose-6-phosphate, which probably acts as an inducer. Therefore, we assume that treR encodes the specific Tre repressor involved in regulation of the expression of the tre operon.
摘要
对枯草芽孢杆菌中包含treP、treA和treR基因的tre位点进行了调控分析。我们证明，至少treP和treA形成一个操纵子，其表达在转录水平受到调控。已在体内和体外研究中对TreR活性进行了研究。treR的插入失活导致treP和treA的组成型表达。在treP上游，我们鉴定出一个248 bp的DNA片段，其中包含一个潜在的依赖于sigmaA的启动子和两个回文序列，这两个回文序列反映了潜在的tre操纵子，在DNA阻滞实验中，它们与含TreR蛋白提取物形成复合物。在海藻糖-6-磷酸存在的情况下，这种复合物的形成被消除，海藻糖-6-磷酸可能作为诱导剂。因此，我们假设treR编码参与tre操纵子表达调控的特异性Tre阻遏物。
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