Tekel'ová D, Repcák M, Zemková E, Tóth J
Planta Med. 2000 Dec;66(8):778-80. doi: 10.1055/s-2000-9779.
Samples of Hyperici herba were obtained by harvesting Hypericum perforatum L. in different plant development stages. The relation of flower development phases in the drug's flower fraction was examined. The HPLC method was then employed for the analysis of the content of secondary metabolites in different flower ontogenesis phases. The content of dianthrones, derivatives of quercetin and hyperforin increased from the first bud phases (0.29%, 0.80%, and 2.47%, respectively) to flowers just opened (1.04%, 4.23% and 6.60%, respectively). The content of dianthrones and quercetin glycosides then decreased (in unripe fruits 0.11% and 0.08%, respectively), whereas the amounts of hyperforin increased to 8.07% in fruits. The content of I3,II8-biapigenin increased from 0.21% in small buds to 1.04% in buds just before opening and has then decreased gradually to a value of 0.02% in fruits. Rutin was not detected in the samples.
通过在不同植物发育阶段采收贯叶连翘来获取金丝桃属植物样本。研究了该药物花部分中花发育阶段的关系。然后采用高效液相色谱法分析不同花个体发育阶段次生代谢产物的含量。二蒽酮、槲皮素衍生物和金丝桃素的含量从最初的芽阶段(分别为0.29%、0.80%和2.47%)增加到刚开放的花(分别为1.04%、4.23%和6.60%)。随后二蒽酮和槲皮素苷的含量下降(未成熟果实中分别为0.11%和0.08%),而金丝桃素的含量在果实中增加到8.07%。I3,II8 - 双芹菜素的含量从小芽中的0.21%增加到即将开放的芽中的1.04%,然后逐渐下降到果实中的0.02%。样本中未检测到芦丁。