Fourneron Jean-Dominique, Naït-Si Youssef
Laboratory of Galenic and Industrial Pharmacy, Faculty of Pharmacy, 27 Boulevard Jean Moulin, 13385 Marseille, France.
Phytochem Anal. 2006 Mar-Apr;17(2):71-7. doi: 10.1002/pca.888.
The effect of the pH of the mobile phase in HPLC analysis of hyperforin was investigated. Working with an extract of St. John's Wort (Hypericum perforatum L.) that is rich in hyperforin, significant differences were observed in conventional chromatograms depending on whether the mobile phase was acidic or alkaline. Chromatogram changes were paralleled by changes in the UV spectrum of the hyperforin peak. The structural changes in hyperforin occur in the chromatographic column itself, as has been confirmed by UV spectroscopy performed on a sample of purified hyperforin, which showed that the UV spectrum is indeed dependent on the pH of its environment.
研究了流动相pH值在金丝桃素高效液相色谱分析中的影响。使用富含金丝桃素的圣约翰草（贯叶连翘）提取物进行实验，根据流动相是酸性还是碱性，常规色谱图中观察到了显著差异。金丝桃素峰的紫外光谱变化与色谱图变化平行。金丝桃素的结构变化发生在色谱柱本身，这已通过对纯化金丝桃素样品进行的紫外光谱分析得到证实，该分析表明紫外光谱确实取决于其所处环境的pH值。
