Department of Biology, University of Padua, via U. Bassi 58/B, I-35131 Padua, Italy.
Fitoterapia. 2010 Mar;81(2):115-9. doi: 10.1016/j.fitote.2009.08.002. Epub 2009 Aug 15.
St. John's Wort extracts are used for the treatment of mild to moderately severe depression, and their composition and standardization have been thoroughly investigated. Standardization of St. John's Wort extracts has to cope with several factors that affect the phytochemical profile of the plant, with geographic location, seasonal variations and subspecies all being important. To address this issue, three different subspecies of Hypericum perforatum L. were profiled in relation to different maturation phases, evaluating the variations in the context of the major secondary metabolites from this plant. HPLC analysis indicated that H. perforatum subsp. perforatum is richer in secondary metabolites than the other subspecies, and that the three subspecies show a different profile during the developmental stages. Hypericins, hyperforins and flavonoids peak at quite different stages, and the standardization of Hyperici extracts based only on the contents of hypericin is not adequate to guarantee batch reproducibility of the extracts.
贯叶金丝桃提取物用于治疗轻度至中度严重的抑郁症，其成分和标准化已经过深入研究。贯叶金丝桃提取物的标准化必须应对影响植物植物化学特征的几个因素，地理位置、季节性变化和亚种都很重要。为了解决这个问题，对三种不同的贯叶金丝桃亚种与不同的成熟阶段进行了分析，评估了主要次生代谢物在这种植物中的变化。HPLC 分析表明，贯叶金丝桃亚种。贯叶金丝桃比其他亚种含有更丰富的次生代谢物，并且在发育阶段这三个亚种表现出不同的特征。金丝桃素、金丝桃素和类黄酮在不同的阶段达到峰值，仅基于金丝桃素含量对贯叶金丝桃提取物进行标准化不足以保证提取物批次的重现性。
