Komorowski R, Grad H, Wu X Y, Friedman S
Department of Endodontics, Faculty of Dentistry, University of Toronto, 124 Edward Street, Toronto, Ontario M5G 1G6, Canada.
J Endod. 2000 Jun;26(6):315-7. doi: 10.1097/00004770-200006000-00001.
Previous studies have demonstrated antimicrobial substantivity in root canal dentin up to 7 days after treatment with chlorhexidine. This in vitro study assessed the antimicrobial substantivity of chlorhexidine-treated bovine root dentin over a period of 21 days. Sixty standardized bovine root sections were randomly divided into three equal groups, and their canals immersed in one of the following solutions: (i) sterile saline; (ii) 2.5% NaOCl; or (iii) 0.2% chlorhexidine (CHX). Half the specimens in each group were treated with the solution for 5 min and the other half for 7 days. After solutions were removed, the specimens were incubated at 37 degrees C in Brain Heart Infusion broth containing Enterococcus faecalis (ATCC 29212). A fresh inoculum was added to the broth every other day over a 21-day period. The canals were then enlarged with sterile burs, and the dentin shavings collected and cultured for the presence of cultivable bacteria in the dentinal tubules. Specimens treated with CHX for 7 days demonstrated significantly less dentin colonization by E. faecalis than the other specimens. CHX has potential as an intracanal medicament, if it can be applied for a period of at least 7 days.
先前的研究表明，用氯己定治疗后，根管牙本质中的抗菌物质可持续长达7天。这项体外研究评估了经氯己定处理的牛牙根牙本质在21天内的抗菌物质残留情况。将60个标准化的牛牙根切片随机分为三组，其根管浸入以下溶液之一：（i）无菌盐水；（ii）2.5%次氯酸钠；或（iii）0.2%氯己定（CHX）。每组中的一半标本用溶液处理5分钟，另一半处理7天。去除溶液后，将标本在含有粪肠球菌（ATCC 29212）的脑心浸液肉汤中于37摄氏度孵育。在21天的时间里，每隔一天向肉汤中添加新鲜接种物。然后用无菌牙钻扩大根管，收集牙本质碎屑并培养，以检测牙本质小管中可培养细菌的存在情况。用CHX处理7天的标本中，粪肠球菌在牙本质中的定植明显少于其他标本。如果氯己定能够至少应用7天，那么它有作为根管内药物的潜力。
