Khademi Abbas Ali, Mohammadi Zahed, Havaee Asgar
Department of Endodontics, Isfahan University of Medical Sciences, Isfahan, Iran.
Aust Endod J. 2006 Dec;32(3):112-5. doi: 10.1111/j.1747-4477.2006.00033.x.
The aim of this in vitro study was to compare the antimicrobial substantivity of 2% chlorhexidine gluconate (CHX), 100 mg ml(-1) doxycycline and 2.6% sodium hypochlorite (NaOCl) in bovine root dentine. Eighty dentine tubes prepared from bovine incisors were infected in vitro for 14 days with Enterococcus faecalis. The specimens were divided into five groups as follows: doxycycline HCl; CHX; NaOCl; infected dentine tubes (positive control); and sterile dentine tubes (negative control). Dentine chips were collected with round burs into tryptic soy broth. After culturing, the number of colony-forming units (CFU) was counted. In all experimental groups, the number of CFU was minimum in the first cultures, and the results obtained were significantly different from each other at any time period (P < 0.05). In the first culture, the NaOCl group and doxycycline HCl group showed the lowest and highest number of CFU, respectively. In each group, the number of CFU increased significantly by time-lapse (P < 0.05). In conclusion, the substantivity of CHX was significantly greater than NaOCl and doxycycline.
本体外研究的目的是比较2%葡萄糖酸氯己定（CHX）、100 mg/ml强力霉素和2.6%次氯酸钠（NaOCl）在牛牙根本质中的抗菌持续性。用牛切牙制备的80根管状牙本质在体外被粪肠球菌感染14天。标本分为以下五组：盐酸强力霉素组；CHX组；NaOCl组；感染的牙本质管（阳性对照）；以及无菌牙本质管（阴性对照）。用圆钻收集牙本质碎屑至胰酪大豆肉汤中。培养后，计算菌落形成单位（CFU）的数量。在所有实验组中，首次培养时CFU数量最少，且在任何时间段获得的结果彼此间均有显著差异（P<0.05）。在首次培养中，NaOCl组和盐酸强力霉素组分别显示出最低和最高的CFU数量。在每组中，CFU数量随时间显著增加（P<0.05）。总之，CHX的持续性显著高于NaOCl和强力霉素。
