Hula N M
Palladin Institute of Biochemistry, NAS of Ukraine.
Ukr Biokhim Zh (1999). 2000 Jul-Oct;72(4-5):21-5.
The review deals with the scientific activity of the Department of Biochemistry of Lipids of the Palladin Biochemistry Institute of the National Academy of Sciences of Ukraine. The estimation of the functional role of some major lipids and of the minor lipid components, namely, N-acylethanolamines (NAE), is the main problem of the scientific investigations of the Department. The role of some lipids in the pathogenesis of diseases accompanied by the oxidative stress was also studied. The Department was the first to find and identify NAE in neuroblastoma C1300 N18 cells. It was shown that NAE with long saturated acyl chains inhibited veratridine-activated fast sodium channels. NAE also activated uterine plasma membrane smooth muscle Ca2+, Mg(2+)-ATPase, and inhibited Fe(2+)-induced free radical oxidation in mitochondria. The results of these investigations served as a basis for development of pharmacological substances with membrane protective properties. It was shown that, at different diseases accompanied by the oxidative stress, a significant change in the lipid composition of cell membranes occurred. Sometimes these changes were adaptive in character, which favored the cell viability adaptation to pathological conditions. The new level of regulation of adaptive reactions can be accompanied by the development of additive injuries of cell viability, which may be caused by the altered level of biologically active cell lipid components. Based on the results of these investigations, the preparation intended for treatment of male infertility was developed. The pharmacological substances intended for treatment of morphine abuse and acute ischemia of myocardium were created.
本综述涉及乌克兰国家科学院帕拉迪恩生物化学研究所脂质生物化学系的科研活动。评估一些主要脂质和次要脂质成分（即N-酰基乙醇胺，NAE）的功能作用是该系科研调查的主要问题。还研究了一些脂质在伴有氧化应激的疾病发病机制中的作用。该系率先在神经母细胞瘤C1300 N18细胞中发现并鉴定了NAE。结果表明，具有长饱和酰基链的NAE抑制藜芦碱激活的快速钠通道。NAE还激活子宫质膜平滑肌Ca2+、Mg(2+)-ATP酶，并抑制线粒体中Fe(2+)诱导的自由基氧化。这些研究结果为开发具有膜保护特性的药理物质奠定了基础。结果表明，在伴有氧化应激的不同疾病中，细胞膜的脂质组成发生了显著变化。有时这些变化具有适应性，有利于细胞活力适应病理状况。适应性反应调节的新水平可能伴随着细胞活力附加损伤的发展，这可能是由生物活性细胞脂质成分水平的改变引起的。基于这些研究结果，开发了用于治疗男性不育症的制剂。研制了用于治疗吗啡成瘾和心肌急性缺血的药理物质。
