Prager R, Mirold S, Tietze E, Strutz U, Knüppel B, Rabsch W, Hardt W D, Tschäpe H
Robert Koch Institut Wernigerode, Germany.
Int J Med Microbiol. 2000 Dec;290(7):605-17. doi: 10.1016/S1438-4221(00)80009-0.
Pathogenic Salmonella enterica strains are capable of causing local and/or systemic infections. They employ two type III secretion systems to translocate an array of virulence-associated proteins (effector proteins) directly into the cytosol of target cells of the host. Earlier data had shown that changes in the repertoire of translocated effector proteins may contribute to the adaptation of Salmonella strains to new hosts and to the emergence of epidemic strains. Using PCR and Southern blot techniques the presence of and the polymorphism among the genes encoding the translocated effector proteins SopB, SopD, SopE, SopE2, SipA, SipB, SipC, AvrA, and SptP was studied in 71 phylogenetically well characterised S. enterica subspecies I (subspecies enterica) strains of the SARB collection and in 209 clinical and epidemic isolates of S. enterica subspecies I belonging to various serovars, phage types, and genotypes. All these Salmonella strains harbour all these respective genes with the exception of sopE and avrA which have been identified in only some of them. Several of the studied genes display genetic polymorphisms (RFLP). These RFLP patterns did not show a strict correlation with the genetic distance, the grouping genes in order to understand their role in the evolution of Salmonella as a pathogen.
致病性肠炎沙门氏菌菌株能够引起局部和/或全身感染。它们利用两种III型分泌系统将一系列与毒力相关的蛋白质（效应蛋白）直接转运到宿主靶细胞的细胞质中。早期数据表明，转运效应蛋白种类的变化可能有助于沙门氏菌菌株适应新宿主以及流行菌株的出现。利用聚合酶链反应（PCR）和Southern印迹技术，对SARB菌株库中71株系统发育特征明确的肠炎沙门氏菌亚种I（肠炎亚种）菌株以及209株属于不同血清型、噬菌体类型和基因型的肠炎沙门氏菌亚种I临床和流行分离株中编码转运效应蛋白SopB、SopD、SopE、SopE2、SipA、SipB、SipC、AvrA和SptP的基因的存在情况及其多态性进行了研究。除了仅在部分菌株中鉴定出的sopE和avrA外，所有这些沙门氏菌菌株都含有所有这些相应的基因。一些研究基因表现出遗传多态性（限制性片段长度多态性，RFLP）。这些RFLP模式与遗传距离没有严格的相关性，对这些基因进行分组以了解它们在沙门氏菌作为病原体进化中的作用。
