血红蛋白E对使用不同商业试剂盒测定糖化血红蛋白的影响。

Effect of hemoglobin E on glycosylated hemoglobin determinations using different commercial kits.

作者信息

Musalmah M, Normah J, Mohamad W B, Salwah O N, Fatah H A, Zahari N A

机构信息

Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan.

出版信息

Med J Malaysia. 2000 Sep;55(3):352-6.

PMID:11200716

Abstract

The effect of HbE, a hemoglobin variant, on the determination of HbA1/HbA1c using 4 commercial kits based on cation-exchange resin, cation-exchange column chromatography and specific antibody techniques was studied. Fifty-eight normal and 63 HbE heterozygous subjects were tested for HbA1 and HbA1c using 4 commercial kits i.e. Eagles Diagnostics, Boehringer Mannehim (BM), Diastat and Ames DCA 2000. Analyses of the samples by the 4 kits were done within one week and samples were stored at 4 degrees C before analysis. The results showed that HbE affects the determination of glycosylated hemoglobin using cation-exchange based and not kits based on specific antibody techniques.

摘要

研究了血红蛋白变异体HbE对使用4种基于阳离子交换树脂、阳离子交换柱色谱法和特异性抗体技术的商业试剂盒测定HbA1/HbA1c的影响。使用4种商业试剂盒，即伊格尔诊断试剂盒、勃林格曼海姆（BM）试剂盒、迪斯塔特试剂盒和艾姆斯DCA 2000试剂盒，对58名正常受试者和63名HbE杂合子受试者的HbA1和HbA1c进行了检测。这4种试剂盒对样本的分析在一周内完成，样本在分析前保存在4摄氏度。结果表明，HbE影响基于阳离子交换的试剂盒测定糖化血红蛋白，而不影响基于特异性抗体技术的试剂盒。