游离核糖体与膜结合核糖体。VI. 兔网织红细胞核糖体中游离巯基的分布。
Free and membrane-bound ribosomes. VI. Distribution of free sulphydryl groups in rabbit reticulocyte ribosomes.
作者信息
Fehlmann M, Dorval M, Godin C
出版信息
Biochim Biophys Acta. 1976 Sep 20;447(1):96-103. doi: 10.1016/0005-2787(76)90099-x.
When free and membrane-bound ribosomes were titrated with N-ethyl[14C]-maleimide or 5,5'-dithio-bis(2-nitrobenzoic acid), 25 free SH groups were detected in polysomes, 10 in the 60 S subunit and 14 in the 40 S subunits. When individual ribosomal proteins were analysed, one large subunit protein L14 was found to be strongly labelled compared to the other ribosomal proteins, in polysomes, monosomes and subunits. However, two additional protein spots from the small subunit were more radioactive in monosomes and subunits than inpolysomes. In all these studies, it was found that the distribution of free SH groups in free and membrane-bound ribosomes was identical except for the few proteins which were reported before to be specific for free or membrane-bound ribosomes (Fehlmann, ., Bellemare, G. and Godin, C. (1975) Biochim. Biophys. Acta 378, 119-124 and Fehlmann, M., Bellemare, G. and Godin, C. (1975) FEBS Lett. 59, 8-12).
当用N-乙基[14C] -马来酰亚胺或5,5'-二硫代双（2-硝基苯甲酸）滴定游离核糖体和膜结合核糖体时，在多核糖体中检测到25个游离巯基，在60S亚基中检测到10个，在40S亚基中检测到14个。当分析单个核糖体蛋白时，发现与其他核糖体蛋白相比，一个大亚基蛋白L14在多核糖体、单核糖体和亚基中被强烈标记。然而，来自小亚基的另外两个蛋白斑点在单核糖体和亚基中比在多核糖体中具有更高的放射性。在所有这些研究中，发现游离核糖体和膜结合核糖体中游离巯基的分布是相同的，除了之前报道的少数对游离核糖体或膜结合核糖体具有特异性的蛋白质（Fehlmann, ., Bellemare, G.和Godin, C. (1975) Biochim. Biophys. Acta 378, 119 - 124以及Fehlmann, M., Bellemare, G.和Godin, C. (1975) FEBS Lett. 59, 8 - 12）。
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