Sousa L G, Petriella A M
Departamento de Ciencias Marinas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3350, 7600, Mar del Plata, Argentina.
Biocell. 2000 Dec;24(3):189-95.
The aim of this study is to describe the histological structure of the hepatopancreas of P. argentinus in intermoult stage in order to provide a baseline for future analysis of its modifications as indicator of environmental stress. Adults at sexual rest of both sexes were collected from Sotelo stream, tributary of Mar Chiquita lagoon, Argentina (38 degrees S 55 degrees W). P. argentinus hepatopancreas was studied by using histological techniques. The hepatopancreas is a bilaterally bilobed brown-yellowish organ. The structure is formed by a mass of blind tubules, with scarce intertubular space. Each tubule consists of a cylindrical epithelial layer surrounded by a basal lamina and myoepithelial cells. Four cellular types were recognized which represent E (embryonic), F (fibrillar), R (resorptive) and B (blisterlike) cells of other decapods. E-cells were found in mitosis and some F-cells had signs of cellular death. B-cells were observed in different phases of holocrine secretion and some groups of desquamated cells appeared at medial and proximal zones, revealing an important cellular turnover rate. According to these observations F, R and B cells are differentiated independently from E-cells.
本研究的目的是描述处于蜕壳间期的阿根廷滑柔鱼肝胰腺的组织学结构，以便为未来将其作为环境胁迫指标的变化分析提供基线。从阿根廷马奇基塔泻湖的支流索特洛河中采集了处于性休止期的成年雌雄个体（南纬38度，西经55度）。采用组织学技术对阿根廷滑柔鱼的肝胰腺进行了研究。肝胰腺是一个双侧双叶的棕黄色器官。其结构由一团盲管组成，管间空间稀少。每个小管由一层圆柱形上皮细胞层组成，周围有基膜和肌上皮细胞。识别出了四种细胞类型，分别代表其他十足类动物的E（胚胎）、F（纤维状）、R（吸收性）和B（泡状）细胞。观察到E细胞处于有丝分裂状态，一些F细胞有细胞死亡的迹象。观察到B细胞处于全浆分泌的不同阶段，在内侧和近端区域出现了一些脱落细胞群，这表明细胞更新率很高。根据这些观察结果，F、R和B细胞是独立于E细胞分化而来的。
