Pandey M, Shukla V K, Singh S, Roy S K, Rao B R
Department of Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
Eur J Cancer Prev. 2000 Dec;9(6):417-22. doi: 10.1097/00008469-200012000-00007.
Gallbladder cancer is a common malignancy among women in India. The aetiology of gallbladder carcinoma is still obscure and although numerous factors have been implicated, none has stood the test of time. This study was carried out to evaluate the role of lipid peroxidation product 4-hydroxynonenal (HNE) in gallbladder carcinogenesis. High-performance liquid chromatography (HPLC) analysis was carried out in 30 bile samples obtained during laparotomy from patients with gallbladder cancer (n = 10), cholelithiasis (n = 10) and age- and sex-matched controls (n = 10). A significantly high concentration of HNE and lower concentration of biliary pH and electrolyte was observed in patients with carcinoma of the gallbladder compared with controls and patients with cholelithiasis. A significant correlation was also observed between biliary Na+ and K+ (r = 0.72), biliary Ca2+ and Na+ (r = 0.69) among controls. However, there was no correlation between HNE, electrolytes and pH among cancer patients. An increased concentration of biliary HNE and decreased concentration of electrolytes suggest biliary stasis rather then increased peroxidation in cancer patients.
胆囊癌是印度女性中常见的恶性肿瘤。胆囊癌的病因仍不明确，尽管有许多因素与之相关，但没有一个经得起时间的考验。本研究旨在评估脂质过氧化产物4-羟基壬烯醛（HNE）在胆囊癌发生中的作用。对30份在剖腹手术中获得的胆汁样本进行了高效液相色谱（HPLC）分析，这些样本来自胆囊癌患者（n = 10）、胆石症患者（n = 10）以及年龄和性别匹配的对照组（n = 10）。与对照组和胆石症患者相比，胆囊癌患者胆汁中HNE浓度显著升高，胆汁pH值和电解质浓度降低。在对照组中，胆汁中Na⁺和K⁺之间（r = 0.72）、胆汁Ca²⁺和Na⁺之间（r = 0.69）也存在显著相关性。然而，癌症患者中HNE、电解质和pH值之间没有相关性。胆汁中HNE浓度升高和电解质浓度降低表明癌症患者存在胆汁淤积而非过氧化增加。
