Kobayashi T, Hirabayashi Y
Supra-Biomolecular System Research Group, Frontier Research System, The Institute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama, Japan.
Glycoconj J. 2000 Mar-Apr;17(3 -4):163-71. doi: 10.1023/a:1026528921085.
Detergent insoluble sphingolipid-cholesterol enriched 'raft'-like membrane microdomains have been implicated in a variety of biological processes including sorting, trafficking, and signaling. Mutant cells and knockout animals of sphingolipid biosynthesis are clearly useful to understand the biological roles of lipid components in raft-like domains. It is suggested that raft-like domains distribute in internal vacuolar membranes as well as plasma membranes. In addition to sphingolipid-cholesterol-rich membrane domains, recent studies suggest the existence of another lipid-membrane domain in the endocytic pathway. This domain is enriched with a unique phospholipid, lysobisphosphatidic acid (LBPA) and localized in the internal membrane of multivesicular endosome. LBPA-rich membrane domains are involved in lipid and protein sorting within the endosomal system. Possible interaction between sphingolipids and LBPA in sphingolipid-storage disease is discussed.
去污剂不溶性富含鞘脂-胆固醇的“筏”样膜微区与多种生物学过程有关，包括分选、运输和信号传导。鞘脂生物合成的突变细胞和基因敲除动物对于理解脂筏样结构域中脂质成分的生物学作用显然很有用。有人提出，筏样结构域分布在内质网膜以及质膜中。除了富含鞘脂-胆固醇的膜结构域外，最近的研究表明在内吞途径中存在另一种脂质-膜结构域。该结构域富含一种独特的磷脂，即溶血双磷脂酸（LBPA），并定位于多泡内体的内膜。富含LBPA的膜结构域参与内体系统中的脂质和蛋白质分选。讨论了鞘脂储存病中鞘脂与LBPA之间可能的相互作用。
