Ley R D
Department of Cell Biology and Physiology, Cancer Research and Treatment Center, University of New Mexico Health Science Center, Albuquerque, NM, USA.
Photochem Photobiol. 2001 Jan;73(1):20-3. doi: 10.1562/0031-8655(2001)073<0020:drfura>2.0.co;2.
Four groups of 30 dorsally shaved opossums (Monodelphis domestica) were exposed to graded doses of ultraviolet radiation A (UVA) (320-400 nm) three times per week for 90 weeks. Animals were monitored for the appearance of focal melanocytic hyperplasia (FMH) and nonmelanoma skin tumors (NMST) during the course of the exposures and for an additional 20 weeks following termination of exposures. FMH is the putative precursor for melanoma in the opossum. The lowest dose of UVA (2.5 x 10(3) J/m2) used in this study was selected based on the action spectrum for the induction of melanoma in a fish model. The prediction was that 2.5 x 10(3) J/m2 would induce FMH in the opossum if the action spectra for the induction of FMH in the opossum and melanoma in the fish were the same. The highest UVA dose was 2.5 x 10(5) J/m2. Only the highest dose of UVA gave a statistically significant induction of FMH and NMST in the opossum. As in previous studies, the FMH appeared earlier than the NMST during the course of exposures and the final prevalence of FMH was lower than the final prevalence of NMST. Overall, the results of this study indicate that the efficacy of UVA to induce FMH in the opossum is not as great as would be predicted from the action spectrum for melanoma induction in a fish model.
将四组30只背部剃毛的负鼠（家短尾负鼠）每周三次暴露于不同剂量的紫外线A（UVA）（320 - 400纳米）下，持续90周。在暴露过程中以及暴露终止后的另外20周内，对动物进行监测，观察局灶性黑素细胞增生（FMH）和非黑素瘤皮肤肿瘤（NMST）的出现情况。FMH被认为是负鼠黑色素瘤的假定前体。本研究中使用的最低UVA剂量（2.5×10³ J/m²）是根据鱼类模型中诱导黑色素瘤的作用光谱选定的。预测是，如果负鼠中诱导FMH的作用光谱与鱼类中诱导黑色素瘤的作用光谱相同，那么2.5×10³ J/m²将在负鼠中诱导FMH。最高UVA剂量为2.5×10⁵ J/m²。只有最高剂量的UVA在负鼠中引起了FMH和NMST的统计学显著诱导。与之前的研究一样，在暴露过程中FMH比NMST出现得更早，并且FMH的最终患病率低于NMST的最终患病率。总体而言，本研究结果表明，UVA在负鼠中诱导FMH的效力不如鱼类模型中诱导黑色素瘤的作用光谱所预测的那样大。
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